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Literature summary for 6.1.1.5 extracted from
- How does Pseudomonas fluorescens avoid suicide from its antibiotic pseudomonic acid?: Evidence for two evolutionarily distinct isoleucyl-tRNA synthetases conferring self-defense (2003), J. Biol. Chem., 278, 25887-25894.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene ileS2, DNA and amino acid sequence determination and analysis, overexpression in Escherichia coli strains DH5alpha and Ts331 mediates resistance against pseudomonic acid, complementation of the ileS-deficient strain Ts331 | Pseudomonas fluorescens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no inhibition of isozyme IleRS-R2 by pseudomonic acid, i.e. muciproin | Pseudomonas fluorescens | |
| pseudomonic acid | i.e. muciproin | Pseudomonas fluorescens |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00125 | - |
L-isoleucine | pH 7.5, recombinant isozyme IleRS-R1 | Pseudomonas fluorescens | |
| 0.007 | - |
ATP | pH 7.5, recombinant isozyme IleRS-R1 | Pseudomonas fluorescens | |
| 0.01 | - |
ATP | pH 7.5, native isozyme IleRS-R2 | Pseudomonas fluorescens | |
| 0.012 | - |
L-isoleucine | pH 7.5, recombinant isozyme IleRS-R2 | Pseudomonas fluorescens | |
| 0.285 | - |
ATP | pH 7.5, recombinant isozyme IleRS-R2 | Pseudomonas fluorescens | |
| 0.33 | - |
ATP | pH 7.5, native isozyme IleRS-R2 | Pseudomonas fluorescens | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-isoleucine + tRNAIle | Pseudomonas fluorescens | - |
AMP + diphosphate + L-isoleucyl-tRNAIle | - |
? | |
| ATP + L-isoleucine + tRNAIle | Pseudomonas fluorescens NCIB 10586 | - |
AMP + diphosphate + L-isoleucyl-tRNAIle | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas fluorescens | - |
gene ileS1, pseudomonic acid-sensitive isozyme IleRS-R1, recombinant enzyme expressed in Escherichia coli | - |
| Pseudomonas fluorescens | Q8L1B1 | pseudomonic acid-resistant isozyme IleRS-R2, gene ileS2 | - |
| Pseudomonas fluorescens NCIB 10586 | - |
gene ileS1, pseudomonic acid-sensitive isozyme IleRS-R1, recombinant enzyme expressed in Escherichia coli | - |
| Pseudomonas fluorescens NCIB 10586 | Q8L1B1 | pseudomonic acid-resistant isozyme IleRS-R2, gene ileS2 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme, and recombinant enzyme from Escherichia coli, amino acid sequence determination | Pseudomonas fluorescens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-isoleucine + tRNAIle | - |
Pseudomonas fluorescens | AMP + diphosphate + L-isoleucyl-tRNAIle | - |
? | |
| ATP + L-isoleucine + tRNAIle | tRNAIle from Pseudomonas fluorescens and Escherichia coli | Pseudomonas fluorescens | AMP + diphosphate + L-isoleucyl-tRNAIle | - |
? | |
| ATP + L-isoleucine + tRNAIle | - |
Pseudomonas fluorescens NCIB 10586 | AMP + diphosphate + L-isoleucyl-tRNAIle | - |
? | |
| ATP + L-isoleucine + tRNAIle | tRNAIle from Pseudomonas fluorescens and Escherichia coli | Pseudomonas fluorescens NCIB 10586 | AMP + diphosphate + L-isoleucyl-tRNAIle | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 117000-117738, native isozyme IleRS-R2, SDS-PAGE and amino acid sequence determination | Pseudomonas fluorescens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| IleRS | - |
Pseudomonas fluorescens |
| IRS | - |
Pseudomonas fluorescens |
| Isoleucine translase | - |
Pseudomonas fluorescens |
| Isoleucine--tRNA ligase | - |
Pseudomonas fluorescens |
| Isoleucine-transfer RNA ligase | - |
Pseudomonas fluorescens |
| Isoleucine-tRNA synthetase | - |
Pseudomonas fluorescens |
| Isoleucyl-transfer ribonucleate synthetase | - |
Pseudomonas fluorescens |
| Isoleucyl-transfer RNA synthetase | - |
Pseudomonas fluorescens |
| Isoleucyl-tRNA synthetase | - |
Pseudomonas fluorescens |
| Mupirocin resistance protein | - |
Pseudomonas fluorescens |
| Synthetase, isoleucyl-transfer ribonucleate | - |
Pseudomonas fluorescens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.18 | - |
L-isoleucine | pH 7.5, recombinant isozyme IleRS-R1 | Pseudomonas fluorescens | |
| 2.64 | - |
L-isoleucine | pH 7.5, recombinant isozyme IleRS-R2 | Pseudomonas fluorescens | |
| 3 | 6 | L-isoleucine | pH 7.5, recombinant isozyme IleRS-R2 | Pseudomonas fluorescens | |
| 3.3 | - |
L-isoleucine | pH 7.5, native isozyme IleRS-R2 | Pseudomonas fluorescens | |
| 4.63 | - |
L-isoleucine | pH 7.5, native isozyme IleRS-R2 | Pseudomonas fluorescens | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Pseudomonas fluorescens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Pseudomonas fluorescens | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0000105 | - |
pseudomonic acid | pH 7.5, recombinant isozyme IleRS-R1, versus L-isoleucine | Pseudomonas fluorescens | |
| 0.000015 | - |
pseudomonic acid | pH 7.5, recombinant isozyme IleRS-R1, versus ATP | Pseudomonas fluorescens | |
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