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Literature summary for 6.1.1.4 extracted from

  • Yan, W.; Tan, M.; Eriani, G.; Wang, E.D.
    Leucine-specific domain modulates the aminoacylation and proofreading functional cycle of bacterial leucyl-tRNA synthetase (2013), Nucleic Acids Res., 41, 4988-4998.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.1.1.4

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Mesomycoplasma mobile

Protein Variants

Protein Variants Comment Organism
K598A the mutation simultaneously reduces the tRNA-binding strength and aminoacylation and editing capacities of the enzyme's leucine-specific domain Mesomycoplasma mobile

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0075
-
 tRNALeu(GAG) in 100 mM Tris-HCl (pH 7.8), 30 mM KCl, 12 mM MgCl2, 5 mM dithiothreitol, at 30°C Mesomycoplasma mobile
0.0076
-
 tRNALeu(UAG) in 100 mM Tris-HCl (pH 7.8), 30 mM KCl, 12 mM MgCl2, 5 mM dithiothreitol, at 30°C Mesomycoplasma mobile

Organism

Organism UniProt Comment Textmining
Mesomycoplasma mobile
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Mesomycoplasma mobile

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-leucine + tRNALeu(GAG)
-
 Mesomycoplasma mobile AMP + diphosphate + L-leucyl-tRNALeu(GAG)
-
 ?
ATP + L-leucine + tRNALeu(UAG)
-
 Mesomycoplasma mobile AMP + diphosphate + L-leucyl-tRNALeu(UAG)
-
 ?

Synonyms

Synonyms Comment Organism
Leucyl-tRNA synthetase
-
 Mesomycoplasma mobile
LeuRS
-
 Mesomycoplasma mobile

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.8
-
 tRNALeu(GAG) in 100 mM Tris-HCl (pH 7.8), 30 mM KCl, 12 mM MgCl2, 5 mM dithiothreitol, at 30°C Mesomycoplasma mobile
2
-
 tRNALeu(UAG) in 100 mM Tris-HCl (pH 7.8), 30 mM KCl, 12 mM MgCl2, 5 mM dithiothreitol, at 30°C Mesomycoplasma mobile

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Mesomycoplasma mobile

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
240
-
 tRNALeu(GAG) in 100 mM Tris-HCl (pH 7.8), 30 mM KCl, 12 mM MgCl2, 5 mM dithiothreitol, at 30°C Mesomycoplasma mobile
260
-
 tRNALeu(UAG) in 100 mM Tris-HCl (pH 7.8), 30 mM KCl, 12 mM MgCl2, 5 mM dithiothreitol, at 30°C Mesomycoplasma mobile