Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Mycobacterium tuberculosis |
Protein Variants | Comment | Organism |
---|---|---|
L949A | the mutant shows 9.5% activity compared to the wild type enzyme | Mycobacterium tuberculosis |
L949K | the mutant shows 2.6% activity compared to the wild type enzyme | Mycobacterium tuberculosis |
L964A | the mutant shows 85% activity compared to the wild type enzyme | Mycobacterium tuberculosis |
L964K | the mutant shows 8.6% activity compared to the wild type enzyme | Mycobacterium tuberculosis |
Q915A | the mutant shows 99% activity compared to the wild type enzyme | Mycobacterium tuberculosis |
Q915K | the mutant shows 54% activity compared to the wild type enzyme | Mycobacterium tuberculosis |
R921A | the mutant shows 37% activity compared to the wild type enzyme | Mycobacterium tuberculosis |
R921K | the mutant shows 83% activity compared to the wild type enzyme | Mycobacterium tuberculosis |
V910A | the mutant shows 90% activity compared to the wild type enzyme | Mycobacterium tuberculosis |
V910P | the mutant shows 3.7% activity compared to the wild type enzyme | Mycobacterium tuberculosis |
V910W | the mutant shows 93% activity compared to the wild type enzyme | Mycobacterium tuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0011 | - |
tRNALeu | mutant enzyme V910A, at pH 8.2 and 30°C | Mycobacterium tuberculosis | |
0.0011 | - |
tRNALeu | wild type enzyme, at pH 8.2 and 30°C | Mycobacterium tuberculosis | |
0.0012 | - |
tRNALeu | mutant enzyme L964A, at pH 8.2 and 30°C | Mycobacterium tuberculosis | |
0.0013 | - |
tRNALeu | mutant enzyme Q915K, at pH 8.2 and 30°C | Mycobacterium tuberculosis | |
0.0014 | - |
tRNALeu | mutant enzyme V910W, at pH 8.2 and 30°C | Mycobacterium tuberculosis | |
0.0018 | - |
tRNALeu | mutant enzyme R921K, at pH 8.2 and 30°C | Mycobacterium tuberculosis | |
0.0019 | - |
tRNALeu | mutant enzyme Q915A, at pH 8.2 and 30°C | Mycobacterium tuberculosis | |
0.0052 | - |
tRNALeu | mutant enzyme R921A, at pH 8.2 and 30°C | Mycobacterium tuberculosis | |
0.0083 | - |
tRNALeu | mutant enzyme L949A, at pH 8.2 and 30°C | Mycobacterium tuberculosis | |
0.0092 | - |
tRNALeu | mutant enzyme V910P, at pH 8.2 and 30°C | Mycobacterium tuberculosis | |
0.0174 | - |
tRNALeu | mutant enzyme L964K, at pH 8.2 and 30°C | Mycobacterium tuberculosis | |
0.0273 | - |
tRNALeu | mutant enzyme L949K, at pH 8.2 and 30°C | Mycobacterium tuberculosis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | the optimum Mg2+:ATP ratio for aminoacylation is 3:1 | Mycobacterium tuberculosis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
108000 | - |
x * 108000, calculated from amino acid sequence | Mycobacterium tuberculosis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-leucine + tRNALeu | Mycobacterium tuberculosis | - |
AMP + diphosphate + L-leucyl-tRNALeu | - |
? | |
ATP + L-leucine + tRNALeu | Mycobacterium tuberculosis H37Rv | - |
AMP + diphosphate + L-leucyl-tRNALeu | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | - |
- |
- |
Mycobacterium tuberculosis H37Rv | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni2+-NTA column chromatography | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-leucine + tRNALeu | - |
Mycobacterium tuberculosis | AMP + diphosphate + L-leucyl-tRNALeu | - |
? | |
ATP + L-leucine + tRNALeu | - |
Mycobacterium tuberculosis H37Rv | AMP + diphosphate + L-leucyl-tRNALeu | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 108000, calculated from amino acid sequence | Mycobacterium tuberculosis |
Synonyms | Comment | Organism |
---|---|---|
Leucyl-tRNA synthetase | - |
Mycobacterium tuberculosis |
LeuRS | - |
Mycobacterium tuberculosis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Mycobacterium tuberculosis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.4 | - |
tRNALeu | mutant enzyme V910P, at pH 8.2 and 30°C | Mycobacterium tuberculosis | |
5 | - |
tRNALeu | mutant enzyme L949K, at pH 8.2 and 30°C | Mycobacterium tuberculosis | |
5 | - |
tRNALeu | mutant enzyme Q915K, at pH 8.2 and 30°C | Mycobacterium tuberculosis | |
5.6 | - |
tRNALeu | mutant enzyme L949A, at pH 8.2 and 30°C | Mycobacterium tuberculosis | |
7 | - |
tRNALeu | mutant enzyme V910A, at pH 8.2 and 30°C | Mycobacterium tuberculosis | |
7.2 | - |
tRNALeu | mutant enzyme L964A, at pH 8.2 and 30°C | Mycobacterium tuberculosis | |
7.8 | - |
tRNALeu | wild type enzyme, at pH 8.2 and 30°C | Mycobacterium tuberculosis | |
9.2 | - |
tRNALeu | mutant enzyme V910W, at pH 8.2 and 30°C | Mycobacterium tuberculosis | |
10.6 | - |
tRNALeu | mutant enzyme L964K, at pH 8.2 and 30°C | Mycobacterium tuberculosis | |
10.6 | - |
tRNALeu | mutant enzyme R921K, at pH 8.2 and 30°C | Mycobacterium tuberculosis | |
13.3 | - |
tRNALeu | mutant enzyme Q915A, at pH 8.2 and 30°C | Mycobacterium tuberculosis | |
13.6 | - |
tRNALeu | mutant enzyme R921A, at pH 8.2 and 30°C | Mycobacterium tuberculosis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.2 | - |
- |
Mycobacterium tuberculosis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Mycobacterium tuberculosis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
183.2 | - |
tRNALeu | mutant enzyme L949K, at pH 8.2 and 30°C | Mycobacterium tuberculosis | |
260.9 | - |
tRNALeu | mutant enzyme V910P, at pH 8.2 and 30°C | Mycobacterium tuberculosis | |
609.2 | - |
tRNALeu | mutant enzyme L964K, at pH 8.2 and 30°C | Mycobacterium tuberculosis | |
674.7 | - |
tRNALeu | mutant enzyme L949A, at pH 8.2 and 30°C | Mycobacterium tuberculosis | |
2615 | - |
tRNALeu | mutant enzyme R921A, at pH 8.2 and 30°C | Mycobacterium tuberculosis | |
3846 | - |
tRNALeu | mutant enzyme Q915K, at pH 8.2 and 30°C | Mycobacterium tuberculosis | |
5889 | - |
tRNALeu | mutant enzyme R921K, at pH 8.2 and 30°C | Mycobacterium tuberculosis | |
6000 | - |
tRNALeu | mutant enzyme L964A, at pH 8.2 and 30°C | Mycobacterium tuberculosis | |
6364 | - |
tRNALeu | mutant enzyme V910A, at pH 8.2 and 30°C | Mycobacterium tuberculosis | |
6571 | - |
tRNALeu | mutant enzyme V910W, at pH 8.2 and 30°C | Mycobacterium tuberculosis | |
7000 | - |
tRNALeu | mutant enzyme Q915A, at pH 8.2 and 30°C | Mycobacterium tuberculosis | |
7091 | - |
tRNALeu | wild type enzyme, at pH 8.2 and 30°C | Mycobacterium tuberculosis |