Activating Compound | Comment | Organism | Structure |
---|---|---|---|
tRNALeu | presence of tRNALeu robustly stimulates editing activity | Escherichia coli | |
tRNALeu | presence of tRNALeu robustly stimulates editing activity | Aquifex aeolicus |
Protein Variants | Comment | Organism |
---|---|---|
T272R | no change in aminoacylation activity, but the deacylation of Ile-tRNALeu is strongly impaired. Mutant still exhibits 45% of wild-type AMP formation | Escherichia coli |
T273R | no change in aminoacylation activity, but the deacylation of Ile-tRNALeu is strongly impaired. Mutant still exhibits 70% of wild-type AMP formation | Aquifex aeolicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5-fluoro-1,3-dihydro-1-hydroxy-2,1-benzoxaborole | i.e. AN2690, 0.1 mM, 5fold decrease in aminoacylation activity | Escherichia coli | |
additional information | aminoacylation and editing reaction are resistant to inactivation by compound AN2690 | Aquifex aeolicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aquifex aeolicus | - |
- |
- |
Escherichia coli | - |
- |
- |