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Literature summary for 6.1.1.4 extracted from

  • Zhu, B.; Yao, P.; Tan, M.; Eriani, G.; Wang, E.
    tRNA-independent pretransfer editing by class I leucyl-tRNA synthetase (2009), J. Biol. Chem., 284, 3418-3424.
    View publication on PubMed
Search for more literature for 6.1.1.4

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Aquifex aeolicus

Protein Variants

Protein Variants Comment Organism
D373A mutant defective in post-transfer editing function Aquifex aeolicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0028
-
 L-leucine 37°C Aquifex aeolicus
0.096
-
 L-norvaline 37°C Aquifex aeolicus
0.698
-
 L-isoleucine 37°C Aquifex aeolicus
0.983
-
 L-methionine 37°C Aquifex aeolicus

Organism

Organism UniProt Comment Textmining
Aquifex aeolicus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-isoleucine + tRNALeu
-
 Aquifex aeolicus AMP + diphosphate + L-isoleucyl-tRNALeu
-
 ?
ATP + L-leucine + tRNALeu
-
 Aquifex aeolicus AMP + diphosphate + L-leucyl-tRNALeu
-
 ?
ATP + L-methionine + tRNALeu
-
 Aquifex aeolicus AMP + diphosphate + L-methionyl-tRNALeu
-
 ?
ATP + L-norvaline + tRNALeu
-
 Aquifex aeolicus AMP + diphosphate + L-norvalyl-tRNALeu
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.9
-
 L-isoleucine 37°C Aquifex aeolicus
3.2
-
 L-methionine 37°C Aquifex aeolicus
12.6
-
 L-norvaline 37°C Aquifex aeolicus
26.3
-
 L-leucine 37°C Aquifex aeolicus

General Information

General Information Comment Organism
physiological function existence of a tRNA-independent pretransfer editing pathway in leucyltRNA synthetases from Aquifex aeolicus. This editing pathway is distinct from the post-transfer editing site and may occur at the synthetic catalytic site Aquifex aeolicus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.7
-
 L-isoleucine 37°C Aquifex aeolicus
3.3
-
 L-methionine 37°C Aquifex aeolicus
13
-
 L-norvaline 37°C Aquifex aeolicus
9400
-
 L-leucine 37°C Aquifex aeolicus