Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Aquifex aeolicus |
Protein Variants | Comment | Organism |
---|---|---|
D373A | mutant defective in post-transfer editing function | Aquifex aeolicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0028 | - |
L-leucine | 37°C | Aquifex aeolicus | |
0.096 | - |
L-norvaline | 37°C | Aquifex aeolicus | |
0.698 | - |
L-isoleucine | 37°C | Aquifex aeolicus | |
0.983 | - |
L-methionine | 37°C | Aquifex aeolicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aquifex aeolicus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-isoleucine + tRNALeu | - |
Aquifex aeolicus | AMP + diphosphate + L-isoleucyl-tRNALeu | - |
? | |
ATP + L-leucine + tRNALeu | - |
Aquifex aeolicus | AMP + diphosphate + L-leucyl-tRNALeu | - |
? | |
ATP + L-methionine + tRNALeu | - |
Aquifex aeolicus | AMP + diphosphate + L-methionyl-tRNALeu | - |
? | |
ATP + L-norvaline + tRNALeu | - |
Aquifex aeolicus | AMP + diphosphate + L-norvalyl-tRNALeu | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.9 | - |
L-isoleucine | 37°C | Aquifex aeolicus | |
3.2 | - |
L-methionine | 37°C | Aquifex aeolicus | |
12.6 | - |
L-norvaline | 37°C | Aquifex aeolicus | |
26.3 | - |
L-leucine | 37°C | Aquifex aeolicus |
General Information | Comment | Organism |
---|---|---|
physiological function | existence of a tRNA-independent pretransfer editing pathway in leucyltRNA synthetases from Aquifex aeolicus. This editing pathway is distinct from the post-transfer editing site and may occur at the synthetic catalytic site | Aquifex aeolicus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.7 | - |
L-isoleucine | 37°C | Aquifex aeolicus | |
3.3 | - |
L-methionine | 37°C | Aquifex aeolicus | |
13 | - |
L-norvaline | 37°C | Aquifex aeolicus | |
9400 | - |
L-leucine | 37°C | Aquifex aeolicus |