Protein Variants | Comment | Organism |
---|---|---|
K600F | the mutation leads to altered catalytic efficiency and perturbations to the discrimination of leucine and isoleucine and affects tRNA recognition and aminoacylation, the mutant demonstrates a 9fold decrease in its ability to distinguish between leucine and isoleucine effectively, the activity is reduced compared to the wild-type enzyme | Homo sapiens |
K600L | the mutation leads to altered catalytic efficiency and perturbations to the discrimination of leucine and isoleucine and affects tRNA recognition and aminoacylation, the mutant demonstrates an 11fold increase in its ability to distinguish between leucine and isoleucine effectively, the activity is reduced compared to the wild-type enzyme | Homo sapiens |
K600R | the mutation leads to altered catalytic efficiency and perturbations to the discrimination of leucine and isoleucine and affects tRNA recognition and aminoacylation, the mutant shows a slight decrease in activity compared to the wild-type enzyme | Homo sapiens |
L570F | the mutation leads to altered catalytic efficiency and perturbations to the discrimination of leucine and isoleucine and affects tRNA recognition and aminoacylation, it shows a catalytic turnover for isoleucine decreased by a factor of 2, L570F has an 11fold higher Km for leucine compared to the wild-type enzyme, the activity is reduceDdcompared to the wild-type enzyme | Escherichia coli |
L570K | the mutation leads to altered catalytic efficiency and perturbations to the discrimination of leucine and isoleucine and affects tRNA recognition and aminoacylation, it shows a catalytic turnover for isoleucine decreased by a factor of 2, L570F has an 11fold higher Km for leucine compared to the wild-type enzyme, the activity is reduced compared to the wild-type enzyme | Escherichia coli |
L570R | the mutation leads to altered catalytic efficiency and perturbations to the discrimination of leucine and isoleucine and affects tRNA recognition and aminoacylation, it shows a catalytic turnover for isoleucine decreased by a factor of 2, L570R has a 4fold stronger binding affinity for leucine compared to the wild-type enzyme, the activity is reduce compared to the wild-type enzyme | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000018 | - |
tRNALeuUUR | human derived substrate, pH 7.0, 37°C, recombinant mutant L570K | Escherichia coli | |
0.00016 | - |
tRNALeuUUR | human derived substrate, pH 7.0, 37°C, recombinant wild-type enzyme | Escherichia coli | |
0.0002 | - |
tRNALeuCUN | Escherichia coli derived substrate, pH 7.0, 37°C, recombinant wild-type enzyme | Homo sapiens | |
0.0004 | - |
tRNALeuCUN | Escherichia coli derived substrate, pH 7.0, 37°C, recombinant wild-type enzyme | Escherichia coli | |
0.0015 | - |
tRNALeuUUR | human derived substrate, pH 7.0, 37°C, recombinant mutant K600L | Homo sapiens | |
0.0017 | - |
tRNALeuCUN | Escherichia coli derived substrate, pH 7.0, 37°C, recombinant mutant L570F | Escherichia coli | |
0.0017 | - |
tRNALeuUUR | human derived substrate, pH 7.0, 37°C, recombinant mutant L570F | Escherichia coli | |
0.0018 | - |
tRNALeuCUN | Escherichia coli derived substrate, pH 7.0, 37°C, recombinant mutants K600L and K600R | Homo sapiens | |
0.002 | - |
tRNALeuCUN | Escherichia coli derived substrate, pH 7.0, 37°C, recombinant mutant L570R | Escherichia coli | |
0.0022 | - |
tRNALeuCUN | Escherichia coli derived substrate, pH 7.0, 37°C, recombinant mutant K600F | Homo sapiens | |
0.004 | - |
tRNALeuUUR | human derived substrate, pH 7.0, 37°C, recombinant mutant K600R | Homo sapiens | |
0.004 | - |
tRNALeuUUR | human derived substrate, pH 7.0, 37°C, recombinant wild-type enzyme | Homo sapiens | |
0.006 | - |
tRNALeuUUR | human derived substrate, pH 7.0, 37°C, recombinant mutant K600F | Homo sapiens | |
0.025 | - |
tRNALeuCUN | Escherichia coli derived substrate, pH 7.0, 37°C, recombinant mutant L570K | Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Homo sapiens | 5739 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Escherichia coli | |
Mg2+ | - |
Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-leucine + tRNALeu | Escherichia coli | - |
AMP + diphosphate + L-leucyl-tRNALeu | - |
? | |
ATP + L-leucine + tRNALeu | Homo sapiens | - |
AMP + diphosphate + L-leucyl-tRNALeu | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Homo sapiens | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Escherichia coli |
additional information | - |
- |
Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-leucine + tRNALeu | - |
Escherichia coli | AMP + diphosphate + L-leucyl-tRNALeu | - |
? | |
ATP + L-leucine + tRNALeu | - |
Homo sapiens | AMP + diphosphate + L-leucyl-tRNALeu | - |
? | |
ATP + L-leucine + tRNALeu | human mitochondrial LeuRS achieves high aminoacylation fidelity without a functional editing active site, representing a rare example of a class I aminoacyl-tRNA synthetase that does not proofread its products, K600 strongly impacts aminoacylation in two ways: it affects both amino acid discrimination and tRNA binding, overview | Homo sapiens | AMP + diphosphate + L-leucyl-tRNALeu | - |
? | |
ATP + L-leucine + tRNALeu | L570 strongly impacts aminoacylation in two ways: it affects both amino acid discrimination and tRNA binding, overview | Escherichia coli | AMP + diphosphate + L-leucyl-tRNALeu | - |
? | |
ATP + L-leucine + tRNALeuCUN | - |
Escherichia coli | AMP + diphosphate + L-leucyl-tRNALeuCUN | - |
? | |
ATP + L-leucine + tRNALeuCUN | - |
Homo sapiens | AMP + diphosphate + L-leucyl-tRNALeuCUN | - |
? | |
ATP + L-leucine + tRNALeuUUR | - |
Escherichia coli | AMP + diphosphate + L-leucyl-tRNALeuUUR | - |
? | |
ATP + L-leucine + tRNALeuUUR | - |
Homo sapiens | AMP + diphosphate + L-leucyl-tRNALeuUUR | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Leucyl-tRNA synthetase | - |
Escherichia coli |
Leucyl-tRNA synthetase | - |
Homo sapiens |
LeuRS | - |
Escherichia coli |
LeuRS | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Escherichia coli |
37 | - |
assay at | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000018 | - |
tRNALeuUUR | human derived substrate, pH 7.0, 37°C, recombinant mutant L570K | Escherichia coli | |
0.00016 | - |
tRNALeuUUR | human derived substrate, pH 7.0, 37°C, recombinant wild-type enzyme | Escherichia coli | |
0.0017 | - |
tRNALeuUUR | human derived substrate, pH 7.0, 37°C, recombinant mutant L570F | Escherichia coli | |
0.003 | - |
tRNALeuUUR | human derived substrate, pH 7.0, 37°C, recombinant mutant K600L | Homo sapiens | |
0.09 | - |
tRNALeuUUR | human derived substrate, pH 7.0, 37°C, recombinant wild-type enzyme | Homo sapiens | |
0.13 | - |
tRNALeuUUR | human derived substrate, pH 7.0, 37°C, recombinant mutant K600F | Homo sapiens | |
0.14 | - |
tRNALeuUUR | human derived substrate, pH 7.0, 37°C, recombinant mutant K600R | Homo sapiens | |
0.35 | - |
tRNALeuCUN | Escherichia coli derived substrate, pH 7.0, 37°C, recombinant mutant K600L | Homo sapiens | |
0.39 | - |
tRNALeuCUN | Escherichia coli derived substrate, pH 7.0, 37°C, recombinant wild-type enzyme | Homo sapiens | |
3 | - |
tRNALeuCUN | Escherichia coli derived substrate, pH 7.0, 37°C, recombinant mutant K600F | Homo sapiens | |
3.2 | - |
tRNALeuCUN | Escherichia coli derived substrate, pH 7.0, 37°C, recombinant mutant K600R | Homo sapiens | |
9.8 | - |
tRNALeuCUN | Escherichia coli derived substrate, pH 7.0, 37°C, recombinant mutant L570K | Escherichia coli | |
14.5 | - |
tRNALeuCUN | Escherichia coli derived substrate, pH 7.0, 37°C, recombinant wild-type enzyme | Escherichia coli | |
21 | - |
tRNALeuCUN | Escherichia coli derived substrate, pH 7.0, 37°C, recombinant mutant L570R | Escherichia coli | |
24 | - |
tRNALeuCUN | Escherichia coli derived substrate, pH 7.0, 37°C, recombinant mutant L570F | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 7.5 | assay at | Escherichia coli |
7 | 7.5 | assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Escherichia coli | |
ATP | - |
Homo sapiens |