Cloned (Comment) | Organism |
---|---|
gene leuS, overexpression in strain JM109(DE3) as His-tagged protein | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0016 | - |
tRNALeu | recombinant and native enzyme | Escherichia coli | |
0.015 | - |
L-leucine | recombinant and native enzyme | Escherichia coli | |
0.26 | - |
ATP | native enzyme | Escherichia coli | |
0.28 | - |
ATP | recombinant enzyme | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
98500 | - |
x * 98500, recombinant enzyme, SDS-PAGE | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-leucine + tRNALeu | Escherichia coli | - |
AMP + diphosphate + L-leucyl-tRNALeu | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from strain JM109(DE3), 4.6fold to homogeneity | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.54 | - |
purified recombinant enzyme | Escherichia coli |
1.69 | - |
purified native enzyme | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-leucine + tRNALeu | - |
Escherichia coli | AMP + diphosphate + L-leucyl-tRNALeu | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 98500, recombinant enzyme, SDS-PAGE | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
Leucine translase | - |
Escherichia coli |
Leucine--tRNA ligase | - |
Escherichia coli |
Leucyl-transfer ribonucleate synthetase | - |
Escherichia coli |
Leucyl-transfer ribonucleic acid synthetase | - |
Escherichia coli |
Leucyl-transfer RNA synthetase | - |
Escherichia coli |
Leucyl-tRNA synthetase | - |
Escherichia coli |
LeuRS | - |
Escherichia coli |
Synthetase, leucyl-transfer ribonucleate | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3 | - |
L-leucine | recombinant enzyme | Escherichia coli | |
3.4 | - |
tRNALeu | recombinant enzyme | Escherichia coli | |
3.4 | - |
L-leucine | native enzyme | Escherichia coli | |
3.6 | - |
ATP | recombinant enzyme | Escherichia coli | |
3.9 | - |
tRNALeu | native enzyme | Escherichia coli | |
4.2 | - |
ATP | native enzyme | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Escherichia coli |