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Literature summary for 6.1.1.4 extracted from
- The beta-subunit of Aquifex aeolicus leucyltRNA synthetase is responsible for cognate tRNA recognition (2002), Biochem. Biophys. Res. Commun., 297, 950-955.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| 2 genes leuS and leuS', overexpression in Escherichia coli BL21(DE3) | Aquifex aeolicus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 35000 | - |
heterodimer in quartenary structure, 2 * 35000, leuS gene product or beta-subunit, + 2 * 65000, leuS gene product or alpha-subunit, SDS-PAGE | Aquifex aeolicus |
| 65000 | - |
heterodimer in quartenary structure, 2 * 35000, leuS gene product or beta-subunit, + 2 * 65000, leuS gene product or alpha-subunit, SDS-PAGE | Aquifex aeolicus |
| 100000 | - |
complexed recombinant leuS' and leuS gene product, i.e. alpha/beta-subunit complex, gel filtration | Aquifex aeolicus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-leucine + tRNALeu | Aquifex aeolicus | - |
AMP + diphosphate + L-leucyl-tRNALeu | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aquifex aeolicus | - |
class I enzyme, 2 genes leuS and leuS' | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzymes from Escherichia coli, leuS' gene product to homogeneity | Aquifex aeolicus |
Renatured (Commentary)
| Renatured (Comment) | Organism |
|---|---|
| renaturation and refolding of recombinant leuS gene product expressed in Escherichia coli | Aquifex aeolicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-leucine + tRNALeu | - |
Aquifex aeolicus | AMP + diphosphate + L-leucyl-tRNALeu | - |
? | |
| ATP + L-leucine + tRNALeu | two-step reaction, the beta-subunit alone is responsible for cognate tRNA recognition, enzyme activity requires both subunits | Aquifex aeolicus | AMP + diphosphate + L-leucyl-tRNALeu | - |
? | |
| additional information | the enzymes forms also perform the reversible ATP-diphosphate exchange reaction, which corresponds to the first reaction step | Aquifex aeolicus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | class I enzyme with unique heterodimeric quartenary structure | Aquifex aeolicus |
| tetramer | heterodimer in quartenary structure, 2 * 35000, leuS' gene product or beta-subunit, + 2 * 65000, leuS gene product or alpha-subunit, SDS-PAGE | Aquifex aeolicus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Leucine translase | - |
Aquifex aeolicus |
| Leucine--tRNA ligase | - |
Aquifex aeolicus |
| Leucyl-transfer ribonucleate synthetase | - |
Aquifex aeolicus |
| Leucyl-transfer ribonucleic acid synthetase | - |
Aquifex aeolicus |
| Leucyl-transfer RNA synthetase | - |
Aquifex aeolicus |
| Leucyl-tRNA synthetase | - |
Aquifex aeolicus |
| LeuRS | - |
Aquifex aeolicus |
| Synthetase, leucyl-transfer ribonucleate | - |
Aquifex aeolicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.9 | - |
assay at | Aquifex aeolicus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Aquifex aeolicus | |
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Release 2023.1 (January 2023)
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