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Literature summary for 6.1.1.4 extracted from

  • Imbault, P.; Sarantoglou, V.; Weil, J.H.
    Properties of purified chloroplastic and cytoplasmic valyl- and leucyl-tRNA synthetases from Euglena gracilis (1982), Phytochemistry, 21, 1189-1193.
No PubMed abstract available
Search for more literature for 6.1.1.4

Activating Compound

Activating Compound Comment Organism Structure
spermidine in absence of Mg2+ spermine allows a very low level of cytoplasmic enzyme Euglena gracilis

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
 Euglena gracilis 9507
-
cytoplasm
-
 Euglena gracilis 5737
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ can partially replace Mg2+ in activation (in chloroplastic enzyme, not in cytoplasmic enzyme) Euglena gracilis
Co2+ can partially replace Mg2+ in activation (in chloroplastic enzyme, not in cytoplasmic enzyme Euglena gracilis
K+ 30 mM KCl enhance activity of cytoplasmic enzyme, no effect on chloroplastic enzyme, concentrations higher than 30 mM inhibit cytoplasmic enzyme more strongly than chloroplastic enzyme Euglena gracilis
Mn2+ can partially replace Mg2+ in activation (in chloroplastic enzyme, not in cytoplasmic enzyme) Euglena gracilis

Organism

Organism UniProt Comment Textmining
Euglena gracilis
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-leucine + tRNALeu chloroplastic enzyme: high specificity towards tRNAs, in contrast the cytoplasmic enzyme recognizes tRNAs from the bleached mutant and from yeast, but also some tRNALeu isoacceptors from E. coli Euglena gracilis AMP + diphosphate + L-leucyl-tRNALeu
-
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