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Literature summary for 6.1.1.4 extracted from

  • Colas, B.; Imbault, P.; Sarantoglou, V.; Boulanger, Y.; Weil, J.H.
    Chloroplastic and cytoplasmic valyl- and leucyl-tRNA synthetases from Euglena gracilis. Comparative study of their structural properties (1982), Biochim. Biophys. Acta, 697, 71-77.
    View publication on PubMed
Search for more literature for 6.1.1.4

General Stability

General Stability Organism
20-30% loss of activity after 1 cycle of freezing and thawing Euglena gracilis
stability is lower with decreasing protein concentration Euglena gracilis

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast
-
 Euglena gracilis 9507
-
cytoplasm
-
 Euglena gracilis 5737
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
100000
-
 1 * 100000, chloroplastic enzyme, SDS-PAGE Euglena gracilis
116000
-
 1 * 116000, cytoplasmic enzyme, SDS-PAGE Euglena gracilis

Organism

Organism UniProt Comment Textmining
Euglena gracilis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Euglena gracilis

Storage Stability

Storage Stability Organism
4°C, 50 mM potassium phosphate buffer, pH 7.0, 1 mM MgCl2, 0.002 mM L-leucine, 100 mM NaCl, 15% v/v 1,2-propanediol, stable for several weeks Euglena gracilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-leucine + tRNALeu
-
 Euglena gracilis AMP + diphosphate + L-leucyl-tRNALeu
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 116000, cytoplasmic enzyme, SDS-PAGE Euglena gracilis
monomer 1 * 100000, chloroplastic enzyme, SDS-PAGE Euglena gracilis