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Literature summary for 6.1.1.4 extracted from
- Purification and properties of leucyl-tRNA synthetase from Candida utilis (1975), Eur. J. Biochem., 57, 169-175.
General Stability
| General Stability | Organism |
|---|---|
| extremely unstable in absence of 2-mercaptoethanol | Cyberlindnera jadinii |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| NEM | - |
Cyberlindnera jadinii |  |
| p-chloromercuribenzoate | - |
Cyberlindnera jadinii | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| K+ | optimal concentration: 80 mM KCl | Cyberlindnera jadinii | |
| Mg2+ | activates | Cyberlindnera jadinii | |
| Mg2+ | optimal concentration: 4 mM (with an ATP concentration of 2 mM) | Cyberlindnera jadinii | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 128000 | - |
1 * 128000, SDS-PAGE | Cyberlindnera jadinii |
| 145000 | - |
disc gel electrophoresis | Cyberlindnera jadinii |
| 150000 | - |
gel filtration | Cyberlindnera jadinii |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cyberlindnera jadinii | - |
Torulopsis | - |
| Cyberlindnera jadinii Torulopsis | - |
Torulopsis | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Cyberlindnera jadinii |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 3.92 | - |
- |
Cyberlindnera jadinii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-leucine + tRNALeu | apart from the homologous substrate the enzyme is able to aminoacylate pure E. coli tRNALeu | Cyberlindnera jadinii | AMP + diphosphate + L-leucyl-tRNALeu | - |
? | |
| ATP + L-leucine + tRNALeu | apart from the homologous substrate the enzyme is able to aminoacylate pure E. coli tRNALeu | Cyberlindnera jadinii Torulopsis | AMP + diphosphate + L-leucyl-tRNALeu | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 128000, SDS-PAGE | Cyberlindnera jadinii |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
half-life: 4.5 min, in absence of 2-mercaptoethanol | Cyberlindnera jadinii |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 23.8 | - |
Leu-tRNALeu | - |
Cyberlindnera jadinii | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Cyberlindnera jadinii |
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Release 2023.1 (January 2023)
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