Cloned (Comment) | Organism |
---|---|
gene thrS, expression of wild-type and mutant enzymes in Escherichia coli strain BL21 | Saccharolobus solfataricus |
gene thrS, expression of wild-type and mutant enzymes in Escherichia coli strain BL21 | Methanocaldococcus jannaschii |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of mutants consisting of catalytic or editing enzyme domains, overview | Methanocaldococcus jannaschii |
additional information | construction of mutants consisting of catalytic or editing enzyme domains, overview, construction of mutant strain PBL205 with a disruption of gene thrS, i.e. SSO3004-3050 | Saccharolobus solfataricus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics of recombinant wild-type and mutant enzymes with threonine and serine | Saccharolobus solfataricus | |
additional information | - |
additional information | kinetics of recombinant wild-type and mutant enzymes with threonine and serine | Methanocaldococcus jannaschii | |
0.1 | - |
L-threonine | pH 7.2, 60°C, recombinant wild-type enzyme | Methanocaldococcus jannaschii | |
0.11 | - |
L-threonine | pH 7.2, 60°C, recombinant wild-type enzyme | Saccharolobus solfataricus | |
25 | - |
L-serine | pH 7.2, 60°C, recombinant wild-type enzyme | Methanocaldococcus jannaschii | |
55 | - |
L-serine | pH 7.2, 60°C, recombinant wild-type enzyme | Saccharolobus solfataricus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Saccharolobus solfataricus | |
Mg2+ | - |
Methanocaldococcus jannaschii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-threonine + tRNAThr | Saccharolobus solfataricus | - |
AMP + diphosphate + L-threonyl-tRNAThr | - |
r | |
ATP + L-threonine + tRNAThr | Methanocaldococcus jannaschii | - |
AMP + diphosphate + L-threonyl-tRNAThr | - |
r | |
additional information | Saccharolobus solfataricus | a freestanding proofreading domain is required for protein synthesis quality control in archaea | ? | - |
? | |
additional information | Methanocaldococcus jannaschii | a freestanding proofreading domain is required for protein synthesis quality control in archaea | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanocaldococcus jannaschii | Q58597 | - |
- |
Saccharolobus solfataricus | Q980D1 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-serine + tRNAThr | - |
Saccharolobus solfataricus | AMP + diphosphate + L-seryl-tRNAThr | - |
? | |
ATP + L-serine + tRNAThr | L-serine is a poor substrate for the wild-type enzyme | Methanocaldococcus jannaschii | AMP + diphosphate + L-seryl-tRNAThr | - |
r | |
ATP + L-threonine + tRNAThr | - |
Saccharolobus solfataricus | AMP + diphosphate + L-threonyl-tRNAThr | - |
r | |
ATP + L-threonine + tRNAThr | - |
Methanocaldococcus jannaschii | AMP + diphosphate + L-threonyl-tRNAThr | - |
r | |
additional information | a freestanding proofreading domain is required for protein synthesis quality control in archaea | Saccharolobus solfataricus | ? | - |
? | |
additional information | a freestanding proofreading domain is required for protein synthesis quality control in archaea | Methanocaldococcus jannaschii | ? | - |
? | |
additional information | L-serine is a poor substrate for the wild-type enzyme, the N-terminal enzyme domain is responsible for editing | Saccharolobus solfataricus | ? | - |
? | |
additional information | the N-terminal enzyme domain is responsible for editing | Methanocaldococcus jannaschii | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | the N-terminal enzyme domain is responsible for editing | Saccharolobus solfataricus |
More | the N-terminal enzyme domain is responsible for editing | Methanocaldococcus jannaschii |
Synonyms | Comment | Organism |
---|---|---|
ThrRS | - |
Saccharolobus solfataricus |
ThrRS | - |
Methanocaldococcus jannaschii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
assay at | Saccharolobus solfataricus |
60 | - |
assay at | Methanocaldococcus jannaschii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.3 | - |
L-serine | pH 7.2, 60°C, recombinant wild-type enzyme | Methanocaldococcus jannaschii | |
1.9 | - |
L-threonine | pH 7.2, 60°C, recombinant wild-type enzyme | Methanocaldococcus jannaschii | |
12.3 | - |
L-serine | pH 7.2, 60°C, recombinant wild-type enzyme | Saccharolobus solfataricus | |
13.5 | - |
L-threonine | pH 7.2, 60°C, recombinant wild-type enzyme | Saccharolobus solfataricus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
assay at | Saccharolobus solfataricus |
7.2 | - |
assay at | Methanocaldococcus jannaschii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
AMP | - |
Saccharolobus solfataricus | |
AMP | - |
Methanocaldococcus jannaschii | |
ATP | - |
Saccharolobus solfataricus | |
ATP | - |
Methanocaldococcus jannaschii |