Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 6.1.1.3 extracted from

  • Korencic, D.; Ahel, I.; Schelert, J.; Sacher, M.; Ruan, B.; Stathopoulos, C.; Blum, P.; Ibba, M.; Soll, D.
    A freestanding proofreading domain is required for protein synthesis quality control in archaea (2004), Proc. Natl. Acad. Sci. USA, 101, 10260-10265.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene thrS, expression of wild-type and mutant enzymes in Escherichia coli strain BL21 Saccharolobus solfataricus
gene thrS, expression of wild-type and mutant enzymes in Escherichia coli strain BL21 Methanocaldococcus jannaschii

Protein Variants

Protein Variants Comment Organism
additional information construction of mutants consisting of catalytic or editing enzyme domains, overview Methanocaldococcus jannaschii
additional information construction of mutants consisting of catalytic or editing enzyme domains, overview, construction of mutant strain PBL205 with a disruption of gene thrS, i.e. SSO3004-3050 Saccharolobus solfataricus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetics of recombinant wild-type and mutant enzymes with threonine and serine Saccharolobus solfataricus
additional information
-
additional information kinetics of recombinant wild-type and mutant enzymes with threonine and serine Methanocaldococcus jannaschii
0.1
-
L-threonine pH 7.2, 60°C, recombinant wild-type enzyme Methanocaldococcus jannaschii
0.11
-
L-threonine pH 7.2, 60°C, recombinant wild-type enzyme Saccharolobus solfataricus
25
-
L-serine pH 7.2, 60°C, recombinant wild-type enzyme Methanocaldococcus jannaschii
55
-
L-serine pH 7.2, 60°C, recombinant wild-type enzyme Saccharolobus solfataricus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Saccharolobus solfataricus
Mg2+
-
Methanocaldococcus jannaschii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-threonine + tRNAThr Saccharolobus solfataricus
-
AMP + diphosphate + L-threonyl-tRNAThr
-
r
ATP + L-threonine + tRNAThr Methanocaldococcus jannaschii
-
AMP + diphosphate + L-threonyl-tRNAThr
-
r
additional information Saccharolobus solfataricus a freestanding proofreading domain is required for protein synthesis quality control in archaea ?
-
?
additional information Methanocaldococcus jannaschii a freestanding proofreading domain is required for protein synthesis quality control in archaea ?
-
?

Organism

Organism UniProt Comment Textmining
Methanocaldococcus jannaschii Q58597
-
-
Saccharolobus solfataricus Q980D1
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-serine + tRNAThr
-
Saccharolobus solfataricus AMP + diphosphate + L-seryl-tRNAThr
-
?
ATP + L-serine + tRNAThr L-serine is a poor substrate for the wild-type enzyme Methanocaldococcus jannaschii AMP + diphosphate + L-seryl-tRNAThr
-
r
ATP + L-threonine + tRNAThr
-
Saccharolobus solfataricus AMP + diphosphate + L-threonyl-tRNAThr
-
r
ATP + L-threonine + tRNAThr
-
Methanocaldococcus jannaschii AMP + diphosphate + L-threonyl-tRNAThr
-
r
additional information a freestanding proofreading domain is required for protein synthesis quality control in archaea Saccharolobus solfataricus ?
-
?
additional information a freestanding proofreading domain is required for protein synthesis quality control in archaea Methanocaldococcus jannaschii ?
-
?
additional information L-serine is a poor substrate for the wild-type enzyme, the N-terminal enzyme domain is responsible for editing Saccharolobus solfataricus ?
-
?
additional information the N-terminal enzyme domain is responsible for editing Methanocaldococcus jannaschii ?
-
?

Subunits

Subunits Comment Organism
More the N-terminal enzyme domain is responsible for editing Saccharolobus solfataricus
More the N-terminal enzyme domain is responsible for editing Methanocaldococcus jannaschii

Synonyms

Synonyms Comment Organism
ThrRS
-
Saccharolobus solfataricus
ThrRS
-
Methanocaldococcus jannaschii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
assay at Saccharolobus solfataricus
60
-
assay at Methanocaldococcus jannaschii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.3
-
L-serine pH 7.2, 60°C, recombinant wild-type enzyme Methanocaldococcus jannaschii
1.9
-
L-threonine pH 7.2, 60°C, recombinant wild-type enzyme Methanocaldococcus jannaschii
12.3
-
L-serine pH 7.2, 60°C, recombinant wild-type enzyme Saccharolobus solfataricus
13.5
-
L-threonine pH 7.2, 60°C, recombinant wild-type enzyme Saccharolobus solfataricus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
assay at Saccharolobus solfataricus
7.2
-
assay at Methanocaldococcus jannaschii

Cofactor

Cofactor Comment Organism Structure
AMP
-
Saccharolobus solfataricus
AMP
-
Methanocaldococcus jannaschii
ATP
-
Saccharolobus solfataricus
ATP
-
Methanocaldococcus jannaschii