Crystallization (Comment) | Organism |
---|---|
enzyme in complex with tRNAThr, hanging drop method, pH 6.5, solution containing ammonium acetate, ATP, and PEG 4000 as precipitant, X-ray diffraction structure determination at 2.9 A resolution and analysis | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | essential, binds at the active site | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-threonine + tRNAThr | Escherichia coli | - |
AMP + diphosphate + L-threonyl-tRNAThr | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
class II enzyme, recombinantly overexpressed and purified enzyme | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + L-threonine + tRNAThr = AMP + diphosphate + L-threonyl-tRNAThr | mechanism, active site structure, and substrate recognition method | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-threonine + tRNAThr | - |
Escherichia coli | AMP + diphosphate + L-threonyl-tRNAThr | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Synthetase, threonyl-transfer ribonucleate | - |
Escherichia coli |
Threonine translase | - |
Escherichia coli |
Threonine--tRNA ligase | - |
Escherichia coli |
Threonine-transfer ribonucleate synthetase | - |
Escherichia coli |
Threonyl-ribonucleic synthetase | - |
Escherichia coli |
Threonyl-transfer ribonucleate synthetase | - |
Escherichia coli |
Threonyl-transfer ribonucleic acid synthetase | - |
Escherichia coli |
Threonyl-transfer RNA synthetase | - |
Escherichia coli |
Threonyl-tRNA synthetase | - |
Escherichia coli |
ThrRS | - |
Escherichia coli |
TRS | - |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Escherichia coli |