KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00045 | - |
tRNAGln | homologous tRNAGln, in absence of regulatory factor beta | Bacillus subtilis | |
0.00065 | - |
tRNAGln | homologous tRNAGln, in presence of regulatory factor beta | Bacillus subtilis | |
0.00083 | - |
tRNAGlu | homologous tRNAGlu, in absence of regulatory factor beta | Bacillus subtilis | |
0.0014 | - |
tRNAGlu | homologous tRNAGlu, in presence of regulatory factor beta | Bacillus subtilis | |
0.0015 | - |
tRNAGln | tRNAGln | Bacillus subtilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-glutamate + tRNAGlx | Bacillus subtilis | the enzyme is responsible for the in vivo aminoacylation of both tRNAGlu and tRNAGln in Bacillus subtilis | AMP + diphosphate + Glu-tRNAGlx | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-glutamate + tRNAGln | the enzyme aminoacylates tRNAGLU and tRNASGln in Bacillus subtilis and efficiently misacylates Escherichia coli tRNA1Gln in vitro | Bacillus subtilis | AMP + diphosphate + Glu-tRNAGln | - |
? | |
ATP + L-glutamate + tRNAGlu | the enzyme aminoacylates tRNAGlu and tRNAsGln in Bacillus subtilis and efficiently misacylates Escherichia coli tRNA1Gln in vitro, not tRNA2Gln from Escherichia coli or tRNAGlu from Escherichia coli | Bacillus subtilis | AMP + diphosphate + Glu-tRNAGlu | - |
? | |
ATP + L-glutamate + tRNAGlu | the enzyme interacts with the G64-C50 or G64-U50 in the Tpsi stem of its tRNA substrate | Bacillus subtilis | AMP + diphosphate + Glu-tRNAGlu | - |
? | |
ATP + L-glutamate + tRNAGlx | the enzyme is responsible for the in vivo aminoacylation of both tRNAGlu and tRNAGln in Bacillus subtilis | Bacillus subtilis | AMP + diphosphate + Glu-tRNAGlx | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
tRNAGln | homologous tRNAGln | Bacillus subtilis | |
0.05 | - |
tRNAGlu | homologous tRNAGlu | Bacillus subtilis | |
0.07 | - |
tRNAGln | tRNAGln from E. coli | Bacillus subtilis |