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Literature summary for 6.1.1.22 extracted from

  • Mladenova, S.R.; Stein, K.R.; Bartlett, L.; Sheppard, K.
    Relaxed tRNA specificity of the Staphylococcus aureus aspartyl-tRNA synthetase enables RNA-dependent asparagine biosynthesis (2014), FEBS Lett., 588, 1808-1812 .
    View publication on PubMed
Search for more literature for 6.1.1.22

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0004
-
 tRNAAsn at pH 7.2 and 37°C Staphylococcus aureus

Organism

Organism UniProt Comment Textmining
Staphylococcus aureus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Staphylococcus aureus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-asparagine + tRNAAsn
-
 Staphylococcus aureus AMP + diphosphate + L-asparaginyl-tRNAAsn
-
 ?
ATP + L-asparagine + tRNAAsn
-
 Staphylococcus aureus FPR3757 AMP + diphosphate + L-asparaginyl-tRNAAsn
-
 ?
additional information no activity with tRNAAsp Staphylococcus aureus ?
-
 ?
additional information no activity with tRNAAsp Staphylococcus aureus FPR3757 ?
-
 ?

Synonyms

Synonyms Comment Organism
AsnRS
-
 Staphylococcus aureus
Asparaginyl-tRNA synthetase
-
 Staphylococcus aureus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.043
-
 tRNAAsn at pH 7.2 and 37°C Staphylococcus aureus

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Staphylococcus aureus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
110
-
 tRNAAsn at pH 7.2 and 37°C Staphylococcus aureus