Protein Variants | Comment | Organism |
---|---|---|
L65A | the mutant strain lys65a produces an enzyme with greater temperature lability in vitro, a higher temperature-independent Km for Asn, and a lower temperature-dependent catalytic capacity than the enzyme from the wild-type strain | Cricetulus griseus |
General Stability | Organism |
---|---|
ATP and Mg2+ stabilize against thermal inactivation | Cricetulus griseus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00006 | - |
tRNAAsn | wild-type and mutant enzyme | Cricetulus griseus | |
0.03 | - |
Asn | wild-type | Cricetulus griseus | |
0.1 | - |
Asn | mutant lys65a | Cricetulus griseus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
152000 | - |
wild-type and mutant enzyme, sucrose density gradient centrifugation | Cricetulus griseus |
155000 | - |
gel filtration | Cricetulus griseus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cricetulus griseus | - |
wild-type and temperature-sensitive mutant strain lys65a | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
ovary | - |
Cricetulus griseus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-asparagine + tRNAAsn | - |
Cricetulus griseus | AMP + diphosphate + L-asparaginyl-tRNAAsn | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
wild-type | Cricetulus griseus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
ATP and Mg2+ stabilize against thermal inactivation | Cricetulus griseus |
37 | - |
wild-type enzyme is stable, mutant enzyme is irreversibly inactivated, half-life: 4.8 min | Cricetulus griseus |