Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 6.1.1.22 extracted from

  • Seignovert, L.; Härtlein, M.; Leberman, R.
    Asparaginyl-tRNA synthetase from Thermus thermophilus HB8. Sequence of the gene and crystallization of the enzyme expressed in Escherichia coli (1996), Eur. J. Biochem., 239, 501-508.
    View publication on PubMed
Search for more literature for 6.1.1.22

Cloned(Commentary)

Cloned (Comment) Organism
overproduced in Escherichia coli Thermus thermophilus

Crystallization (Commentary)

Crystallization (Comment) Organism
-
 Thermus thermophilus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
 high similarity with other bacterial asparaginyl-tRNA synthetase sequences (30-55% identity) Thermus thermophilus
50000
-
 x * 50875, calculation from nucleotide sequence, x * 50000, SDS-PAGE Thermus thermophilus
50875
-
 x * 50875, calculation from nucleotide sequence, x * 50000, SDS-PAGE Thermus thermophilus

Organism

Organism UniProt Comment Textmining
Thermus thermophilus
-
 expressed in Escherichia coli
-
Thermus thermophilus HB8 / ATCC 27634 / DSM 579
-
 expressed in Escherichia coli
-

Purification (Commentary)

Purification (Comment) Organism
-
 Thermus thermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-asparagine + tRNAAsn
-
 Thermus thermophilus AMP + diphosphate + L-asparaginyl-tRNAAsn
-
 ?
ATP + L-asparagine + tRNAAsn
-
 Thermus thermophilus HB8 / ATCC 27634 / DSM 579 AMP + diphosphate + L-asparaginyl-tRNAAsn
-
 ?

Subunits

Subunits Comment Organism
? x * 50875, calculation from nucleotide sequence, x * 50000, SDS-PAGE Thermus thermophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
85
-
 21 h, retains 50% of its activity Thermus thermophilus
90
-
 1 h, retains 50% of its activity Thermus thermophilus