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Literature summary for 6.1.1.21 extracted from

  • Augustine, J.; Francklyn, C.
    Design of an active fragment of a class II aminoacyl-tRNA synthetase and its significance for synthetase evolution (1997), Biochemistry, 36, 3473-3482.
    View publication on PubMed
Search for more literature for 6.1.1.21

Protein Variants

Protein Variants Comment Organism
NcatHisRS 320-residue fragment, NcatHisRS, truncated immediately following motif 3 catalyzes both the the specific aminoacylation of tRNA and ATP-diphosphate exchange, albeit less efficiently than the full length enzyme. NcatHisRs shows no mischarging of noncognate tRNAs but exhibits reduced selectivity for the C73 discriminator base. NcatHisRs is monomeric, indicating that the C-terminal domain is essential for maintaining the dimeric structure of the enzyme Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00031
-
 U73tRNAHisGUG tuncated enzyme form NcatHisRS, aminoacylation Escherichia coli
0.00056
-
 tRNAHis truncated enzyme form NcatHisRS, aminoacylation Escherichia coli
0.0014
-
 tRNAHis full-length HisRS, aminoacylation Escherichia coli
0.0016
-
 U73tRNAHisGUG full-length HisRS, aminoacylation Escherichia coli
0.0055
-
 tRNAHisCUA tuncated enzyme form NcatHisRS, aminoacylation Escherichia coli
0.013
-
 tRNAHisCUA full-length HisRS, aminoacylation Escherichia coli
0.03
-
 His wild-type HisRS, ATP-diphosphate exchange Escherichia coli
0.046
-
 His tuncated enzyme form NcatHisRS, ATP-diphosphate exchange Escherichia coli
0.74
-
 ATP truncated enzyme form NcatHisRS, ATP-diphosphate exchange Escherichia coli
0.89
-
 ATP wild-type HisRS, ATP-diphosphate exchange Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
 full-length enzyme and a 320-residue fragment (NcatHisRS) truncated immediately following motif 3
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-histidine + tRNAHis also active with tRNAHisCUA, very weak activity with U73tRNAHisGUG Escherichia coli AMP + diphosphate + L-histidyl-tRNAHis
-
 ?
ATP + L-histidine + tRNAHisCUA
-
 Escherichia coli AMP + diphosphate + L-histidyl-tRNAHisCUA
-
 ?
ATP + L-histidine + U73tRNAHisGUG
-
 Escherichia coli AMP + diphosphate + L-histidyl-U73tRNAHisGUG
-
 ?
ATP + L-histidine + wild type tRNAHis
-
 Escherichia coli AMP + diphosphate + L-histidyl-tRNAHis
-
 ?
additional information ATP-diphosphate exchange Escherichia coli ?
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.005
-
 U73tRNAHisGUG full-length HisRS, aminoacylation Escherichia coli
0.016
-
 U73tRNAHisGUG truncated enzyme form NcatHisRS, aminoacylation Escherichia coli
0.17
-
 ATP truncated enzyme form NcatHisRS, ATP-diphosphate exchange Escherichia coli
0.19
-
 His truncated enzyme form NcatHisRS, ATP-diphosphate exchange Escherichia coli
0.26
-
 tRNAHisCUA truncated enzyme form NcatHisRS, aminoacylation Escherichia coli
0.64
-
 wild type tRNAHis truncated enzyme form NcatHisRS, aminoacylation Escherichia coli
1.5
-
 tRNAHisCUA full-length HisRS, aminoacylation Escherichia coli
2.6
-
 wild type tRNAHis full-length HisRS, aminoacylation Escherichia coli
120
-
 ATP wild-type HisRS, ATP-diphosphate exchange Escherichia coli
142
-
 His wild-type HisRS, ATP-diphosphate exchange Escherichia coli