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Literature summary for 6.1.1.20 extracted from
- Identification, cloning, and expression of a functional phenylalanyl-tRNA synthetase (pheRS) from Staphylococcus aureus (2001), Protein Expr. Purif., 21, 470-484.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| genes pheS and pheT encoding the alpha and beta subunits in one transcriptional operon, DNA and amino acid sequence determination and analysis, overexpression of both genes in an artificial operon in Escherichia coli | Staphylococcus aureus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00094 | - |
L-phenylalanine | recombinant enzyme, pH 7.9, 22°C | Staphylococcus aureus |  |
| 0.0012 | - |
L-phenylalanine | native enzyme, pH 7.9, 22°C | Staphylococcus aureus | |
| 0.122 | - |
ATP | recombinant enzyme, pH 7.9, 22°C | Staphylococcus aureus | |
| 0.147 | - |
ATP | native enzyme, pH 7.9, 22°C | Staphylococcus aureus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40000 | - |
alpha-subunit of recombinant enzyme, mass spectroscopy | Staphylococcus aureus |
| 89090 | - |
beta-subunit of recombinant enzyme, mass spectroscopy | Staphylococcus aureus |
| 90000 | - |
2 * 40000, alpha subunit, + 2 * 90000, beta-subunit, (alphabeta)2, recombinant enzyme, SDS-PAGE | Staphylococcus aureus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-phenylalanine + tRNAPhe | Staphylococcus aureus | - |
AMP + diphosphate + L-phenylalanyl-tRNAPhe | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Staphylococcus aureus | - |
strain WCUH29 and Oxford strain, genes pheS and pheT | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme from Oxford strain, 50% purity, recombinant enzyme overexpressed in Escherichia coli, over 90% purity | Staphylococcus aureus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.074 | - |
purified native enzyme | Staphylococcus aureus |
| 0.12 | - |
purified recombinant enzyme | Staphylococcus aureus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-phenylalanine + tRNAPhe | - |
Staphylococcus aureus | AMP + diphosphate + L-phenylalanyl-tRNAPhe | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 2 * 40000, alpha subunit, + 2 * 90000, beta-subunit, (alphabeta)2, recombinant enzyme, SDS-PAGE | Staphylococcus aureus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CML33 | - |
Staphylococcus aureus |
| FRS | - |
Staphylococcus aureus |
| HSPC173 | - |
Staphylococcus aureus |
| L-Phenylalanyl-tRNA synthetase | - |
Staphylococcus aureus |
| Phenylalanine translase | - |
Staphylococcus aureus |
| Phenylalanine--tRNA ligase | - |
Staphylococcus aureus |
| Phenylalanine-tRNA synthetase | - |
Staphylococcus aureus |
| Phenylalanyl transfer ribonucleic acid synthetase | - |
Staphylococcus aureus |
| Phenylalanyl-transfer ribonucleate synthetase | - |
Staphylococcus aureus |
| Phenylalanyl-transfer RNA ligase | - |
Staphylococcus aureus |
| Phenylalanyl-transfer RNA synthetase | - |
Staphylococcus aureus |
| Phenylalanyl-tRNA ligase | - |
Staphylococcus aureus |
| Phenylalanyl-tRNA synthetase | - |
Staphylococcus aureus |
| PheRS | - |
Staphylococcus aureus |
| Synthetase, phenylalanyl-transfer ribonucleate | - |
Staphylococcus aureus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 22 | - |
assay at | Staphylococcus aureus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.9 | - |
assay at | Staphylococcus aureus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | a 15fold excess is required for optimal activity | Staphylococcus aureus | |
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