Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 6.1.1.2 extracted from

  • Seshaiah, P.; Andrew, D.J.
    WRS-85D: A tryptophanyl-tRNA synthetase expressed to high levels in the developing Drosophila salivary gland (1999), Mol. Biol. Cell, 10, 1595-1608.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
the house-keeping gene maps to the cytological position 85D and is therefore termed WRS-85D, expression is dependent on the homeotic gene Sex combs reduced, i.e. Scr, ectopic expression of Scr results in expression of WRS-85D in new locations, recombinant expression in Escherichia coli strain BL21(DE3) as His-tagged enzyme Drosophila melanogaster

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-tryptophan + tRNATrp Drosophila melanogaster enzyme is required for embryonic survival, and is essential for viability of the fly AMP + diphosphate + L-tryptophan-tRNATrp
-
?

Organism

Organism UniProt Comment Textmining
Drosophila melanogaster Q9U4Y0 single copy gene
-
Drosophila melanogaster Q9U4Y1 single copy gene
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme form Escherichia coli strain BL21(DE3) Drosophila melanogaster

Source Tissue

Source Tissue Comment Organism Textmining
embryo
-
Drosophila melanogaster
-
larva
-
Drosophila melanogaster
-
additional information enzyme-deficiency is lethal, accumulation pattern after treatment with interferons alpha, beta, and gamma Drosophila melanogaster
-
salivary gland embryonic, developing Drosophila melanogaster
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-tryptophan + tRNATrp wild-type and recombinant enzyme are specific for tRNATrp Drosophila melanogaster AMP + diphosphate + L-tryptophan-tRNATrp
-
?
ATP + L-tryptophan + tRNATrp enzyme is required for embryonic survival, and is essential for viability of the fly Drosophila melanogaster AMP + diphosphate + L-tryptophan-tRNATrp
-
?

Synonyms

Synonyms Comment Organism
TrpRS
-
Drosophila melanogaster
Tryptophanyl-tRNA synthase
-
Drosophila melanogaster

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Drosophila melanogaster

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Drosophila melanogaster

Cofactor

Cofactor Comment Organism Structure
ATP
-
Drosophila melanogaster