Cloned (Comment) | Organism |
---|---|
the house-keeping gene maps to the cytological position 85D and is therefore termed WRS-85D, expression is dependent on the homeotic gene Sex combs reduced, i.e. Scr, ectopic expression of Scr results in expression of WRS-85D in new locations, recombinant expression in Escherichia coli strain BL21(DE3) as His-tagged enzyme | Drosophila melanogaster |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-tryptophan + tRNATrp | Drosophila melanogaster | enzyme is required for embryonic survival, and is essential for viability of the fly | AMP + diphosphate + L-tryptophan-tRNATrp | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Drosophila melanogaster | Q9U4Y0 | single copy gene | - |
Drosophila melanogaster | Q9U4Y1 | single copy gene | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme form Escherichia coli strain BL21(DE3) | Drosophila melanogaster |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
embryo | - |
Drosophila melanogaster | - |
larva | - |
Drosophila melanogaster | - |
additional information | enzyme-deficiency is lethal, accumulation pattern after treatment with interferons alpha, beta, and gamma | Drosophila melanogaster | - |
salivary gland | embryonic, developing | Drosophila melanogaster | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-tryptophan + tRNATrp | wild-type and recombinant enzyme are specific for tRNATrp | Drosophila melanogaster | AMP + diphosphate + L-tryptophan-tRNATrp | - |
? | |
ATP + L-tryptophan + tRNATrp | enzyme is required for embryonic survival, and is essential for viability of the fly | Drosophila melanogaster | AMP + diphosphate + L-tryptophan-tRNATrp | - |
? |
Synonyms | Comment | Organism |
---|---|---|
TrpRS | - |
Drosophila melanogaster |
Tryptophanyl-tRNA synthase | - |
Drosophila melanogaster |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Drosophila melanogaster |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Drosophila melanogaster |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Drosophila melanogaster |