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Literature summary for 6.1.1.2 extracted from
- Identification and characterization of human mitochondrial tryptophanyl-tRNA synthetase (2000), J. Biol. Chem., 275, 16820-16826.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| isozyme hmtTrpRS, DNA and amino acid sequence determination and analysis, expression as C-terminally His-tagged enzyme in Spodoptera frugiperda Sf9 and HighFive BTI-NT-5B1-4 insect cells via baculovirus infection, expression as GFP-fusion protein in human 293 cells | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.027 | - |
L-tryptophan | pH 9.0, 22°C, recombinant enzyme, exchange assay | Homo sapiens |  |
| 0.4 | - |
ATP | pH 9.0, 22°C, recombinant enzyme, exchange assay | Homo sapiens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | - |
Homo sapiens | 5739 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 35000 | - |
x * 35000, recombinant His-tagged enzyme, expressed in insect cells, SDS-PAGE | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-tryptophan + tRNATrp | Homo sapiens | - |
AMP + diphosphate + L-tryptophan-tRNATrp | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | Q9UGM6 | mitochondrial specific isozyme containing a mitochondrial localization signal sequence | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant from insect cells | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-tryptophan + tRNATrp | - |
Homo sapiens | AMP + diphosphate + L-tryptophan-tRNATrp | - |
? | |
| L-tryptophan + ATP | the enzyme also catalyzes the exchange of diphosphate in the diphosphate-ATP exchange assay | Homo sapiens | L-Trp-adenylate + diphosphate | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 35000, recombinant His-tagged enzyme, expressed in insect cells, SDS-PAGE | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| TrpRS | - |
Homo sapiens |
| Tryptophanyl-tRNA synthase | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 22 | - |
exchange assay at, room temperature | Homo sapiens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.2 | - |
L-tryptophan | pH 9.0, 22°C, recombinant enzyme, exchange assay | Homo sapiens | |
| 1.4 | - |
ATP | pH 9.0, 22°C, recombinant enzyme, exchange assay | Homo sapiens | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
exchange assay at | Homo sapiens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Homo sapiens | |
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