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Literature summary for 6.1.1.2 extracted from

  • Jia, J.; Xu, F.; Chen, X.; Chen, L.; Jin, Y.; Wang, D.T.
    Two essential regions for tRNA recognition in Bacillus subtilis tryptophanyl-tRNA synthetase (2002), Biochem. J., 365, 749-756.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressionof wild-type and mutant enzymes in Escherichia coli strain JM109 Bacillus subtilis

Protein Variants

Protein Variants Comment Organism
additional information construction of deletion mutants with decreased activity by deletion of residues 108-122, causing loss of 44% activity, or 234-238, causing loss of 80% activity, or both parts, resulting in an inactive mutant enzyme, deletion of residues 234-238 affected the normally induced expression at 37°C Bacillus subtilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00018
-
tRNATrp pH 7.5, 22°C, wild-type enzyme Bacillus subtilis
0.00022
-
tRNATrp pH 7.5, 22°C, deletion 108-122 mutant Bacillus subtilis
0.00052
-
tRNATrp pH 7.5, 22°C, deletion 234-238 mutant Bacillus subtilis
0.00073
-
L-tryptophan pH 7.5, 22°C, wild-type enzyme Bacillus subtilis
0.0021
-
L-tryptophan pH 7.5, 22°C, deletion 108-122 mutant Bacillus subtilis
0.0023
-
L-tryptophan pH 7.5, 22°C, deletion 234-238 mutant Bacillus subtilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-tryptophan + tRNATrp Bacillus subtilis
-
AMP + diphosphate + L-tryptophan-tRNATrp
-
?

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme and mutants from Escherichia coli JM109, to homogeneity Bacillus subtilis

Reaction

Reaction Comment Organism Reaction ID
ATP + L-tryptophan + tRNATrp = AMP + diphosphate + L-tryptophyl-tRNATrp 2-step reaction, tRNATrp substrate recognition, mechanism and modeling Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-tryptophan + tRNATrp
-
Bacillus subtilis AMP + diphosphate + L-tryptophan-tRNATrp
-
?
ATP + L-tryptophan + tRNATrp 2-step reaction, tRNATrp substrate recognition, mechanism and modeling Bacillus subtilis AMP + diphosphate + L-tryptophan-tRNATrp
-
?
additional information enzyme also performs the ATP-diphosphate exchange reaction Bacillus subtilis ?
-
?

Synonyms

Synonyms Comment Organism
TrpRS
-
Bacillus subtilis
Tryptophanyl-tRNA synthase
-
Bacillus subtilis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
assay at Bacillus subtilis
25
-
exchange reaction assay at Bacillus subtilis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.59
-
L-tryptophan pH 7.5, 22°C, deletion 234-238 mutant Bacillus subtilis
0.59
-
tRNATrp pH 7.5, 22°C, deletion 234-238 mutant Bacillus subtilis
0.61
-
L-tryptophan pH 7.5, 22°C, deletion 108-122 mutant Bacillus subtilis
0.61
-
tRNATrp pH 7.5, 22°C, deletion 108-122 mutant Bacillus subtilis
0.9
-
L-tryptophan pH 7.5, 22°C, wild-type enzyme Bacillus subtilis
0.9
-
tRNATrp pH 7.5, 22°C, wild-type enzyme Bacillus subtilis
6.08
-
L-tryptophan pH 7.5, 22°C, deletion 108-122 mutant Bacillus subtilis
6.08
-
tRNATrp pH 7.5, 22°C, deletion 108-122 mutant Bacillus subtilis
6.08
-
L-tryptophan pH 7.5, 22°C, deletion 234-238 mutant Bacillus subtilis
6.08
-
tRNATrp pH 7.5, 22°C, deletion 234-238 mutant Bacillus subtilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Bacillus subtilis

Cofactor

Cofactor Comment Organism Structure
ATP
-
Bacillus subtilis