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Literature summary for 6.1.1.18 extracted from

  • Sathyapriya, R.; Vishveshwara, S.
    Structure networks of E. coli glutaminyl-tRNA synthetase: effects of ligand binding (2007), Proteins, 68, 541-550.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
E222K site-directed mutagenesis, mutational structure-function study, the residue is part of the invariant Hub, the mutation leads to mischarging and affected cognate tRNAGln recognition Escherichia coli
F90L site-directed mutagenesis, mutational structure-function study, the residue is part of the connection in the active site network, the mutant shows increased Glu recognition in vitro and in vivo Escherichia coli
Q255I site-directed mutagenesis, mutational structure-function study, the residue is part of the invariant Hub, the mutation leads to reduced specificity for cognate Gln recognition and increased Glu recognition Escherichia coli
R341A site-directed mutagenesis, mutational structure-function study, the residue is part of the Hub common to all liganded complex, the mutation affects anticodon recognition Escherichia coli
Y211H site-directed mutagenesis, mutational structure-function study, the residue is part of the connection in the quaternary cognate-complex, the mutants shows slow solvation dynamics in the active site Escherichia coli
Y240E/G site-directed mutagenesis, mutational structure-function study, the residue is part of the Hub common to ligand-free and quaternary cognate-complex, the mutant shows increased Glu recognition in vitro and in vivo Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-glutamine + tRNAGln Escherichia coli
-
AMP + diphosphate + L-glutaminyl-tRNAGln
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli P00962
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-glutamine + tRNAGln
-
Escherichia coli AMP + diphosphate + L-glutaminyl-tRNAGln
-
?
additional information ternary complexed GlnRS bound to tRNAGln and the Gln-AMP analogue is catalytically active and has undergone the first step of the aminoacylation reaction Escherichia coli ?
-
?

Subunits

Subunits Comment Organism
More GlnRS structure networks, detection method development for accounting side chain interactions, yet providing a global view of the ligand-induced conformational changes, and understand allosteric changes mediated by the binding of ligands, usage of crystal structures: PDB IDs 1nyl, 1qtq, 1o0b, and 1o0c, overview Escherichia coli

Synonyms

Synonyms Comment Organism
Glutaminyl-tRNA synthetase
-
Escherichia coli

Cofactor

Cofactor Comment Organism Structure
ATP
-
Escherichia coli