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Literature summary for 6.1.1.18 extracted from
- The glutaminyl-transfer RNA synthetase of Escherichia coli. Purification, structure and function relationship (1980), Biochim. Biophys. Acta, 607, 65-80.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| spermine | significant stimulation of ATP-diphosphate exchange and aminoacylation in presence of limited MgCl2 concentrations, cannot totally replace Mg2+ | Escherichia coli |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 5,5'-dithiobis(2-nitrobenzoate) | - |
Escherichia coli | |
| p-hydroxymercuribenzoate | - |
Escherichia coli | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0002 | - |
tRNAGln | - |
Escherichia coli | |
| 0.15 | - |
Gln | - |
Escherichia coli | |
| 0.21 | - |
ATP | - |
Escherichia coli | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | can partially replace Mg2+ in activation of ATP-diphosphate exchange | Escherichia coli | |
| Mg2+ | required | Escherichia coli | |
| Mg2+ | optimal MgCl2/ATP ratio is 2-5 at 2 mM ATP in aminoacylation | Escherichia coli | |
| Mn2+ | can replace Mg2+ in activation of aminoacylation | Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 65400 | 71200 | sucrose density gradient sedimentation | Escherichia coli |
| 68500 | - |
electrophoresis of the native enzyme in polyacrylamide gels of various concentrations | Escherichia coli |
| 69000 | - |
1 * 69000, SDS-PAGE | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.28 | - |
- |
Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-glutamine + tRNAGln | - |
Escherichia coli | AMP + diphosphate + L-glutaminyl-tRNAGln | - |
? | |
| additional information | lack of tRNA-independent diphosphate exchange | Escherichia coli | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 69000, SDS-PAGE | Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
- |
Escherichia coli |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3.3 | - |
tRNAGln | - |
Escherichia coli | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.2 | - |
ATP-diphosphate exchange | Escherichia coli |
| 8.5 | - |
aminoacylation | Escherichia coli |
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Release 2023.1 (January 2023)
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