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Literature summary for 6.1.1.16 extracted from
- Kinetic quality control of anticodon recognition by a eukaryotic aminoacyl-tRNA synthetase (2007), J. Mol. Biol., 367, 1063-1078.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene cysS, stable expression of C-terminally His-tagged wild-type and mutant enzymes in Escherichia coli | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | construction of a DELTAC mutant of the enzyme that terminates at V642, removing residues 643 to 748 from the full-length enzyme, the mutant enzymes shows increased activity in the first amino acid activation step and reduced activity in the second aminoacylation step compared to the wild-type full-length enzyme | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | steady-state, single turnover, and multiple turnover kinetics of recombinant His-tagged wild-type full-length enzyme, and recombinant His-tagged wild-type deletion mutant, kinetic analysis of the two reaction steps, overview | Homo sapiens | |
| 0.0009 | - |
tRNACys | recombinant His-tagged wild-type full-length enzyme, amino acid activation step | Homo sapiens |  |
| 0.0045 | - |
tRNACys | recombinant His-tagged deletion mutant, amino acid activation step | Homo sapiens | |
| 0.0103 | - |
L-cysteine | recombinant His-tagged deletion mutant, amino acid activation step | Homo sapiens | |
| 0.0203 | - |
L-cysteine | recombinant His-tagged wild-type full-length enzyme, amino acid activation step | Homo sapiens | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 76000 | - |
2 * 87000, recombinant His-tagged wild-type full-length enzyme, SDS-PAGE, 2 * 76000, recombinant His-tagged deletion mutant enzymes, SDS-PAGE | Homo sapiens |
| 87000 | - |
2 * 87000, recombinant His-tagged wild-type full-length enzyme, SDS-PAGE, 2 * 76000, recombinant His-tagged deletion mutant enzymes, SDS-PAGE | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-cysteine + tRNACys | Homo sapiens | - |
AMP + diphosphate + L-cysteinyl-tRNACys | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
gene cysS | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant C-terminally His-tagged wild-type and mutant enzymes from Escherichia coli to homogeneity | Homo sapiens |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-cysteine + tRNACys | - |
Homo sapiens | AMP + diphosphate + L-cysteinyl-tRNACys | - |
? | |
| ATP + L-cysteine + tRNACys | a two-step process of amino acid activation as first step, and aminocylation as second step | Homo sapiens | AMP + diphosphate + L-cysteinyl-tRNACys | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 87000, recombinant His-tagged wild-type full-length enzyme, SDS-PAGE, 2 * 76000, recombinant His-tagged deletion mutant enzymes, SDS-PAGE | Homo sapiens |
| More | human CysRS does not associate with the multi-synthetase complex, yet contains an eukaryotic-specific C-terminal extension that follows the tRNA anticodon-binding domain, the C-terminal extension is not involved in dimer formation, but is important for stable binding of tRNACys in the aminoacylation reaction step | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CysRS | - |
Homo sapiens |
| Cysteinyl-tRNA synthetase | - |
Homo sapiens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.2 | - |
tRNACys | recombinant His-tagged deletion mutant, amino acid activation step | Homo sapiens | |
| 2.3 | - |
tRNACys | recombinant His-tagged wild-type full-length enzyme, amino acid activation step | Homo sapiens | |
| 10.3 | - |
L-cysteine | recombinant His-tagged wild-type full-length enzyme, amino acid activation step | Homo sapiens | |
| 18.1 | - |
L-cysteine | recombinant His-tagged deletion mutant, amino acid activation step | Homo sapiens | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Homo sapiens | |
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