Any feedback?
Literature summary for 6.1.1.16 extracted from
- Influence of transfer RNA tertiary structure on aminoacylation efficiency by glutaminyl and cysteinyl-tRNA synthetases (2000), J. Mol. Biol., 299, 431-446.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression in strain JM109 | Escherichia coli |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | tRNAGln mutant substrates | Escherichia coli | |
| 0.0015 | - |
tRNACys | pH 7.0, 37°C | Escherichia coli |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-cysteine + tRNACys | Escherichia coli | - |
AMP + diphosphate + L-cysteinyl-tRNACys | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant from overexpressing strain JM109 | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-cysteine + tRNACys | - |
Escherichia coli | AMP + diphosphate + L-cysteinyl-tRNACys | - |
? | |
| ATP + L-cysteine + tRNACys mutant | tRNA substrate is a tRNACys with mutation at the core tertiary Levitt pair from wild-type G15.C48 to mutant G15.G48, leading to a higher activity | Escherichia coli | AMP + diphosphate + L-cysteinyl-tRNACys mutant | - |
? | |
| ATP + L-cysteine + tRNAGln duoble-mutant | tRNA substrate is a tRNAGln with introduced tRNACys indentitiy nucleotides at the acceptor and anticodon ends and a core tertiary Levitt pair equivalent to tRNAGln of G15.G48, poor activity | Escherichia coli | AMP + diphosphate + L-cysteinyl-tRNAGln double-mutant | - |
? | |
| ATP + L-cysteine + tRNAGln mutant | tRNA substrate is a tRNAGln with introduced tRNACys indentitiy nucleotides at the acceptor and anticodon ends and a core tertiary Levitt pair equivalent to tRNACys of G15.C48 | Escherichia coli | AMP + diphosphate + L-cysteinyl-tRNAGln mutant | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CysRS | - |
Escherichia coli |
| Cysteine translase | - |
Escherichia coli |
| Cysteine--tRNA ligase | - |
Escherichia coli |
| Cysteinyl-transfer ribonucleate synthetase | - |
Escherichia coli |
| Cysteinyl-transfer RNA synthetase | - |
Escherichia coli |
| Cysteinyl-tRNA synthetase | - |
Escherichia coli |
| Synthetase, cysteinyl-transfer ribonucleate | - |
Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Escherichia coli |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | tRNAGln mutant substrates | Escherichia coli | |
| 3 | 6 | tRNACys | pH 7.0, 37°C | Escherichia coli | |
| 3.47 | - |
tRNACys | pH 7.0, 37°C | Escherichia coli | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Escherichia coli |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Escherichia coli | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
