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Literature summary for 6.1.1.16 extracted from

  • Burnell, J.N.; Shrift, A.
    Cysteinyl-tRNA synthetase from Phaseolus aureus. Purification and properties (1977), Plant Physiol., 60, 670-674.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.1.1.16

Inhibitors

Inhibitors Comment Organism Structure
ATP free ATP inhibits Vigna radiata var. radiata
D-cysteine
-
 Vigna radiata var. radiata
EDTA
-
 Vigna radiata var. radiata
glutathione reduced Vigna radiata var. radiata
NaN3
-
 Vigna radiata var. radiata
NEM
-
 Vigna radiata var. radiata
O-acetylserine
-
 Vigna radiata var. radiata
p-chloromercuribenzoate
-
 Vigna radiata var. radiata
phenylhydrazine
-
 Vigna radiata var. radiata
S2-
-
 Vigna radiata var. radiata

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.05
-
 selenocysteine ATP-diphosphate exchange Vigna radiata var. radiata
0.062
-
 Cys ATP-diphosphate exchange Vigna radiata var. radiata
1.15
-
 ATP ATP-diphosphate exchanhge Vigna radiata var. radiata
10
-
 2-aminobutyric acid ATP-diphosphate exchange Vigna radiata var. radiata

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 15% of the efficiency of Mg2+ in activation of ATP-diphosphate exchange Vigna radiata var. radiata
Mg2+ required for ATP-diphosphate exchange Vigna radiata var. radiata
Mg2+ optimal Mg2+/ATP ratio is 2.5 Vigna radiata var. radiata
Mn2+ 9% of the efficiency of Mg2+ in activation of ATP-diphosphate exchange Vigna radiata var. radiata

Organism

Organism UniProt Comment Textmining
Vigna radiata var. radiata
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Vigna radiata var. radiata

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Vigna radiata var. radiata

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 2-aminobutyric acid + tRNACys
-
 Vigna radiata var. radiata AMP + diphosphate + L-alpha-aminobutyryl-tRNACys
-
 ?
ATP + L-cysteine + tRNACys
-
 Vigna radiata var. radiata AMP + diphosphate + L-cysteinyl-tRNACys
-
 ?
ATP + selenocysteine + tRNACys
-
 Vigna radiata var. radiata AMP + diphosphate + selenocysteinyl-tRNACys
-
 ?
additional information ATP-diphosphate exchange Vigna radiata var. radiata ?
-
 ?
additional information selenocysteine can replace cysteine in ATP-diphosphate exchange Vigna radiata var. radiata ?
-
 ?
additional information 2-aminobutyric acid can replace cysteine in ATP-diphosphate exchange Vigna radiata var. radiata ?
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 ATP-diphosphate exchange Vigna radiata var. radiata

pH Range

pH Minimum pH Maximum Comment Organism
7 9 pH 7.0: about 30% of maximal activity, pH 9.0: about 50% of maximal activity, ATP-diphosphate exchange Vigna radiata var. radiata