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Literature summary for 6.1.1.16 extracted from

  • Komatsoulis, G.A.; Abelson, J.
    Recognition of tRNACys by Escherichia coli cysteinyl-tRNA synthetase [published erratum appears in Biochemistry 1993 Dec 7;32(48):13374] (1993), Biochemistry, 32, 7435-7444.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
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additional information Km-values of mutant tRNA molecules Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-cysteine + tRNACys tRNACys mutants: greatest loss of activity caused by mutation of a single nucleotide, occurs when the discriminator U73 is changed, mutations in the wobble nucleotide of the anticodon also cause reductions in the specificity constant of 3 orders of magnitude, while mutations in the other anticodon nucleotides causes lesser effects Escherichia coli AMP + diphosphate + L-cysteinyl-tRNACys
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Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
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additional information turnover numbers of mutant tRNA molecules Escherichia coli