KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Km-values of mutant tRNA molecules | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-cysteine + tRNACys | tRNACys mutants: greatest loss of activity caused by mutation of a single nucleotide, occurs when the discriminator U73 is changed, mutations in the wobble nucleotide of the anticodon also cause reductions in the specificity constant of 3 orders of magnitude, while mutations in the other anticodon nucleotides causes lesser effects | Escherichia coli | AMP + diphosphate + L-cysteinyl-tRNACys | - |
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Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | turnover numbers of mutant tRNA molecules | Escherichia coli |