Cloned (Comment) | Organism |
---|---|
expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Rhodopseudomonas palustris |
expression of the enzyme in Escherichia coli strain M15 | Enterococcus faecalis |
Crystallization (Comment) | Organism |
---|---|
purified recombinant His-tagged wild-type and selenomethionine-labeled enzymes, hanging drop vapour diffusion method, mixing of protein solution containing 5 mg/ml with an equal volume of the reservoir solution containing 0.1 M citric acid, pH 5.5, 15%-17% PEG 3000, cryoprotection by 20% ethylene glycol, for PrsRp-adenylate analog complex cocrystals, the enzyme is mixed first with 500 nM ProAMS or 100 nM CysAMS, and then with an equal volume of a solution containing 0.1 M citric acid, pH 5.5, 10%-11% PEG 3000, 15%-20% ethylene glycol, X-ray diffraction structure determination and analysis at 2.9 A resolution | Rhodopseudomonas palustris |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5'-O-(N-[prolyl]-sulphamoyl) adenosine | i.e. ProAMS, a nonhydrolyzable analogue of the prolyl-adenylate | Enterococcus faecalis | |
5'-O-(N-[prolyl]-sulphamoyl) adenosine | i.e. ProAMS, a nonhydrolyzable analogue of the prolyl-adenylate | Rhodopseudomonas palustris |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Enterococcus faecalis | |
Mg2+ | - |
Rhodopseudomonas palustris |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-proline + tRNAPro | Enterococcus faecalis | - |
AMP + diphosphate + L-prolyl-tRNAPro | - |
? | |
ATP + L-proline + tRNAPro | Rhodopseudomonas palustris | - |
AMP + diphosphate + L-prolyl-tRNAPro | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Enterococcus faecalis | Q831W7 | - |
- |
Rhodopseudomonas palustris | Q6N5P6 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli strain M15 by ammonium sulfate fractionation and anion exchange chromatography | Enterococcus faecalis |
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Rhodopseudomonas palustris |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-proline + tRNAPro | - |
Enterococcus faecalis | AMP + diphosphate + L-prolyl-tRNAPro | - |
? | |
ATP + L-proline + tRNAPro | - |
Rhodopseudomonas palustris | AMP + diphosphate + L-prolyl-tRNAPro | - |
? | |
ATP + L-proline + tRNAPro | small-substrate recognition by the prokaryote-type ProRS, model for posttransfer editing conformation, overview | Enterococcus faecalis | AMP + diphosphate + L-prolyl-tRNAPro | - |
? | |
ATP + L-proline + tRNAPro | small-substrate recognition by the prokaryote-type ProRS, model for posttransfer editing conformation, overview | Rhodopseudomonas palustris | AMP + diphosphate + L-prolyl-tRNAPro | - |
? | |
additional information | comparison of the overall enzyme structure and binding mode of ATP and prolyl-adenylate with those of the archael/eukaryote-type ProRS from Thermus thermophilus, overview | Enterococcus faecalis | ? | - |
? | |
additional information | comparison of the overall enzyme structure and binding mode of ATP and prolyl-adenylate with those of the archael/eukaryote-type ProRS from Thermus thermophilus, overview | Rhodopseudomonas palustris | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | comparison of the overall enzyme structure and binding mode of ATP and prolyl-adenylate with those of the archael/eukaryote-type ProRS from Thermus thermophilus, cognate and noncognate adenylate analogue complexes, overview | Rhodopseudomonas palustris |
More | comparison of the overall enzyme structure and binding mode of ATP and prolyl-adenylate with those of the archael/eukaryote-type ProRS from Thermus thermophilus, overview | Enterococcus faecalis |
Synonyms | Comment | Organism |
---|---|---|
Prolyl-tRNA synthetase | - |
Enterococcus faecalis |
Prolyl-tRNA synthetase | - |
Rhodopseudomonas palustris |
ProRS | - |
Enterococcus faecalis |
ProRS | - |
Rhodopseudomonas palustris |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | binding structure and complex formation with cognate tRNA, overview | Enterococcus faecalis | |
ATP | binding structure and complex formation with cognate tRNA, overview | Rhodopseudomonas palustris |