Literature summary for 6.1.1.15 extracted from

Ambrogelly, A.; Ahel, I.; Polycarpo, C.; Bunjun-Srihari, S.; Krett, B.; Jacquin-Becker, C.; Ruan, B.; Kohrer, C.; Stathopoulos, C.; RajBhandary, U.L.; Soll, D.
Methanocaldococcus jannaschii prolyl-tRNA synthetase charges tRNA(Pro) with cysteine (2002), J. Biol. Chem., 277, 34749-34754.

Search for more literature for 6.1.1.15

Cloned(Commentary)

Cloned (Comment)	Organism
overexpression of wild-type and C-terminally truncated enzymes as His-tagged proteins in Escherichia coli	Methanocaldococcus jannaschii

Protein Variants

Protein Variants	Comment	Organism
additional information	the C-terminally truncated enzyme is 3fold less active with L-cysteine and 10fold less active with L-alanine compared too the wild-type enzyme	Methanocaldococcus jannaschii

Inhibitors

Inhibitors	Comment	Organism	Structure
cysteamine	inhibition of Cys-tRNAPro formation	Methanocaldococcus jannaschii
D-cysteine-DL-homocysteine	inhibition of Cys-tRNAPro formation	Methanocaldococcus jannaschii
L-cysteine ethyl ester	inhibition of Cys-tRNAPro formation	Methanocaldococcus jannaschii
L-cysteine methyl ester	inhibition of Cys-tRNAPro formation	Methanocaldococcus jannaschii
additional information	no inhibition of Cys-tRNAPro formation by L-sulfinic acid, L-cysteic acid, S-methyl L-cysteine, and homocysteine thiolactone	Methanocaldococcus jannaschii
N-acetyl-L-cysteine	inhibition of Cys-tRNAPro formation	Methanocaldococcus jannaschii

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + L-proline + tRNAPro	Methanocaldococcus jannaschii	-	AMP + diphosphate + L-prolyl-tRNAPro	-	?

Organism

Organism	UniProt	Comment	Textmining
Methanocaldococcus jannaschii	-	gene proS	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His-tagged wild-type and C-terminally truncated enzymes from overexpressing Escherichia coli	Methanocaldococcus jannaschii

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + L-alanine + tRNAPro	misacetylation of tRNAPro, no editing	Methanocaldococcus jannaschii	AMP + diphosphate + L-alanyl-tRNAPro	-	?
ATP + L-cysteine + tRNAPro	misacetylation of tRNAPro, no editing	Methanocaldococcus jannaschii	AMP + diphosphate + L-cysteinyl-tRNAPro	-	?
ATP + L-proline + tRNAPro	-	Methanocaldococcus jannaschii	AMP + diphosphate + L-prolyl-tRNAPro	-	?
additional information	enzyme does not perform deacetylation reaction of mischarged Cys-tRNAPro and Ala-tRNAPro, the enzyme also performs the ATP-diphosphate exchange reaction, no charging of tRNACys with L-cysteine	Methanocaldococcus jannaschii	?	-	?

Synonyms

Synonyms	Comment	Organism
Global RNA synthesis factor	-	Methanocaldococcus jannaschii
Pro-tRNA synthetase	-	Methanocaldococcus jannaschii
Proline translase	-	Methanocaldococcus jannaschii
Proline--tRNA ligase	-	Methanocaldococcus jannaschii
Prolyl RNA synthetase	-	Methanocaldococcus jannaschii
Prolyl-transfer ribonucleate synthetase	-	Methanocaldococcus jannaschii
Prolyl-transfer ribonucleic acid synthetase	-	Methanocaldococcus jannaschii
Prolyl-transfer RNA synthetase	-	Methanocaldococcus jannaschii
Prolyl-tRNA synthetase	-	Methanocaldococcus jannaschii
ProRS	-	Methanocaldococcus jannaschii

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
50	-	assay at	Methanocaldococcus jannaschii

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7	-	aminoacylation assay at	Methanocaldococcus jannaschii
7.2	-	ATP-diphosphate exchange assay at	Methanocaldococcus jannaschii

Cofactor

Cofactor	Comment	Organism	Structure
ATP	-	Methanocaldococcus jannaschii

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Release 2023.1 (January 2023)