Cloned (Comment) | Organism |
---|---|
overexpression of wild-type and C-terminally truncated enzymes as His-tagged proteins in Escherichia coli | Methanocaldococcus jannaschii |
Protein Variants | Comment | Organism |
---|---|---|
additional information | the C-terminally truncated enzyme is 3fold less active with L-cysteine and 10fold less active with L-alanine compared too the wild-type enzyme | Methanocaldococcus jannaschii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
cysteamine | inhibition of Cys-tRNAPro formation | Methanocaldococcus jannaschii | |
D-cysteine-DL-homocysteine | inhibition of Cys-tRNAPro formation | Methanocaldococcus jannaschii | |
L-cysteine ethyl ester | inhibition of Cys-tRNAPro formation | Methanocaldococcus jannaschii | |
L-cysteine methyl ester | inhibition of Cys-tRNAPro formation | Methanocaldococcus jannaschii | |
additional information | no inhibition of Cys-tRNAPro formation by L-sulfinic acid, L-cysteic acid, S-methyl L-cysteine, and homocysteine thiolactone | Methanocaldococcus jannaschii | |
N-acetyl-L-cysteine | inhibition of Cys-tRNAPro formation | Methanocaldococcus jannaschii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-proline + tRNAPro | Methanocaldococcus jannaschii | - |
AMP + diphosphate + L-prolyl-tRNAPro | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanocaldococcus jannaschii | - |
gene proS | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged wild-type and C-terminally truncated enzymes from overexpressing Escherichia coli | Methanocaldococcus jannaschii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-alanine + tRNAPro | misacetylation of tRNAPro, no editing | Methanocaldococcus jannaschii | AMP + diphosphate + L-alanyl-tRNAPro | - |
? | |
ATP + L-cysteine + tRNAPro | misacetylation of tRNAPro, no editing | Methanocaldococcus jannaschii | AMP + diphosphate + L-cysteinyl-tRNAPro | - |
? | |
ATP + L-proline + tRNAPro | - |
Methanocaldococcus jannaschii | AMP + diphosphate + L-prolyl-tRNAPro | - |
? | |
additional information | enzyme does not perform deacetylation reaction of mischarged Cys-tRNAPro and Ala-tRNAPro, the enzyme also performs the ATP-diphosphate exchange reaction, no charging of tRNACys with L-cysteine | Methanocaldococcus jannaschii | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Global RNA synthesis factor | - |
Methanocaldococcus jannaschii |
Pro-tRNA synthetase | - |
Methanocaldococcus jannaschii |
Proline translase | - |
Methanocaldococcus jannaschii |
Proline--tRNA ligase | - |
Methanocaldococcus jannaschii |
Prolyl RNA synthetase | - |
Methanocaldococcus jannaschii |
Prolyl-transfer ribonucleate synthetase | - |
Methanocaldococcus jannaschii |
Prolyl-transfer ribonucleic acid synthetase | - |
Methanocaldococcus jannaschii |
Prolyl-transfer RNA synthetase | - |
Methanocaldococcus jannaschii |
Prolyl-tRNA synthetase | - |
Methanocaldococcus jannaschii |
ProRS | - |
Methanocaldococcus jannaschii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
assay at | Methanocaldococcus jannaschii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
aminoacylation assay at | Methanocaldococcus jannaschii |
7.2 | - |
ATP-diphosphate exchange assay at | Methanocaldococcus jannaschii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Methanocaldococcus jannaschii |