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Literature summary for 6.1.1.14 extracted from

  • Dignam, J.D.; Qu, X.; Chaires, J.B.
    Equilibrium unfolding of Bombyx mori glycyl-tRNA synthetase (2001), J. Biol. Chem., 276, 4028-4037.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type and N-terminally truncated enzyme, the latter lacking 55 N-terminal amino acid residues, in Escherichia coli BL21(DE3) Bombyx mori

Inhibitors

Inhibitors Comment Organism Structure
additional information reversible denaturation by urea or guanidine-HCl, wild-type enzyme and N-terminally truncated mutant both dissociate to monomers and exhibit a cooperative transition at higher denaturant concentration, wild-type unfolds by a multistate process and 2 parallel state unfoldings, which are absent in the truncated mutant Bombyx mori

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + glycine + tRNAGly Bombyx mori
-
AMP + diphosphate + glycyl-tRNAGly
-
?

Organism

Organism UniProt Comment Textmining
Bombyx mori
-
-
-

Renatured (Commentary)

Renatured (Comment) Organism
reversible denaturation by urea or guanidine-HCl, wild-type enzyme and N-terminally truncated mutant both dissociate to monomers and exhibit a cooperative transition at higher denaturant concentration, wild-type unfolds by a multistate process and 2 parallel state unfoldings, which are absent in the truncated mutant Bombyx mori

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + glycine + tRNAGly
-
Bombyx mori AMP + diphosphate + glycyl-tRNAGly
-
?

Synonyms

Synonyms Comment Organism
Glycine--tRNA ligase
-
Bombyx mori
Glycyl translase
-
Bombyx mori
Glycyl-transfer ribonucleate synthetase
-
Bombyx mori
Glycyl-transfer ribonucleic acid synthetase
-
Bombyx mori
Glycyl-transfer RNA synthetase
-
Bombyx mori
Glycyl-tRNA synthetase
-
Bombyx mori
GlyRS
-
Bombyx mori
Synthetase, glycyl-transfer ribonucleate
-
Bombyx mori

Cofactor

Cofactor Comment Organism Structure
ATP
-
Bombyx mori