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Literature summary for 6.1.1.14 extracted from

  • Dignam, S.S.; Dignam, J.D.
    Glycyl- and alanyl-tRNA synthetases from Bombyx mori. Purification and properties (1984), J. Biol. Chem., 259, 4043-4048.
    View publication on PubMed

General Stability

General Stability Organism
sensitive to repeated freezing and thawing Bombyx mori
unstable after dilution to less than 0.02 mg/ml in absence of nonspecific carrier proteins Bombyx mori

Inhibitors

Inhibitors Comment Organism Structure
spermine
-
Bombyx mori

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0025
-
tRNAGly substrate from liver Bombyx mori
0.0033
-
tRNAGly substrate from Bombyx mori Bombyx mori
0.18
-
Gly
-
Bombyx mori
0.37
-
ATP
-
Bombyx mori

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Bombyx mori
Mg2+ optimal concentration is 3-5 mM Bombyx mori
additional information the enzyme is twice as active in Tris-HCl or Hepes-NaOH as it is in potassium phosphate buffer Bombyx mori

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
80000
-
2 * 80000, SDS-PAGE Bombyx mori
160000
-
gel filtration, sedimentation velocity Bombyx mori

Organism

Organism UniProt Comment Textmining
Bombyx mori
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Bombyx mori

Source Tissue

Source Tissue Comment Organism Textmining
silk gland posterior Bombyx mori
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Bombyx mori

Storage Stability

Storage Stability Organism
-80°C, retains approximately 90% of its activity after 3 months Bombyx mori

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + glycine + tRNAGly
-
Bombyx mori AMP + diphosphate + glycyl-tRNAGly
-
?

Subunits

Subunits Comment Organism
dimer 2 * 80000, SDS-PAGE Bombyx mori

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
additional information
-
Bombyx mori

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 8.5
-
Bombyx mori