General Stability | Organism |
---|---|
sensitive to repeated freezing and thawing | Bombyx mori |
unstable after dilution to less than 0.02 mg/ml in absence of nonspecific carrier proteins | Bombyx mori |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
spermine | - |
Bombyx mori |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0025 | - |
tRNAGly | substrate from liver | Bombyx mori | |
0.0033 | - |
tRNAGly | substrate from Bombyx mori | Bombyx mori | |
0.18 | - |
Gly | - |
Bombyx mori | |
0.37 | - |
ATP | - |
Bombyx mori |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Bombyx mori | |
Mg2+ | optimal concentration is 3-5 mM | Bombyx mori | |
additional information | the enzyme is twice as active in Tris-HCl or Hepes-NaOH as it is in potassium phosphate buffer | Bombyx mori |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
80000 | - |
2 * 80000, SDS-PAGE | Bombyx mori |
160000 | - |
gel filtration, sedimentation velocity | Bombyx mori |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bombyx mori | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Bombyx mori |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
silk gland | posterior | Bombyx mori | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Bombyx mori |
Storage Stability | Organism |
---|---|
-80°C, retains approximately 90% of its activity after 3 months | Bombyx mori |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + glycine + tRNAGly | - |
Bombyx mori | AMP + diphosphate + glycyl-tRNAGly | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 80000, SDS-PAGE | Bombyx mori |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Bombyx mori |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 8.5 | - |
Bombyx mori |