KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.13 | - |
diphosphate | ATP-diphosphate exchange reaction | Lactiplantibacillus plantarum | |
2.5 | - |
ATP | formation of D-alanine hydroamate | Lactiplantibacillus plantarum | |
40 | - |
D-Ala | formation of D-alanine hydroxamate | Lactiplantibacillus plantarum | |
70 | - |
D-Ala | ATP-diphosphate exchange reaction | Lactiplantibacillus plantarum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lactiplantibacillus plantarum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial | Lactiplantibacillus plantarum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.5 | - |
- |
Lactiplantibacillus plantarum |
Storage Stability | Organism |
---|---|
-10°C, ammonium sulfate fraction stable for 6 months | Lactiplantibacillus plantarum |
-20°C, chromatographed fraction loses 25% of activity in 1 week | Lactiplantibacillus plantarum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-Ala + hydroxylamine | D-Ala can be substituted by D-2-amino-n-butyric acid with 14.6% and D-Ser with 7.5% efficiency | Lactiplantibacillus plantarum | D-Alanine hydroxamate + AMP + diphosphate | - |
? | |
ATP + diphosphate | - |
Lactiplantibacillus plantarum | ATP + diphosphate | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 8 | substrates D-Ala + hydroxylamine + ATP | Lactiplantibacillus plantarum |
7.5 | 8 | ATP-diphosphate exchange reaction | Lactiplantibacillus plantarum |