Cloned (Comment) | Organism |
---|---|
expression in Saccharomyces cerevisiae, and expression as His-tagged protein | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of several N-terminal deletion mutants with altered mRNA binding properties, overview | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-aspartate + tRNAAsp | Saccharomyces cerevisiae | - |
AMP + diphosphate + L-aspartyl-tRNAAsp | - |
? | |
additional information | Saccharomyces cerevisiae | the enzyme also binds to its mRNA via its N-terminal extension of 70 amino acid residues and the anticodon-binding module, which has a regulatory function on the expression of the enzyme | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
dimeric class II aspartyl-tRNA synthetase | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + L-aspartate + tRNAAsp = AMP + diphosphate + L-aspartyl-tRNAAsp | RNA-binding motif XSKXXLKKXK | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-aspartate + tRNAAsp | - |
Saccharomyces cerevisiae | AMP + diphosphate + L-aspartyl-tRNAAsp | - |
? | |
additional information | the enzyme also binds to its mRNA via its N-terminal extension of 70 amino acid residues and the anticodon-binding module, which has a regulatory function on the expression of the enzyme | Saccharomyces cerevisiae | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | modular structure | Saccharomyces cerevisiae |
More | the N-terminal 70 amino acid residues extension protrudes from the anticodon-binding module, it is not essential for acylation activity but contains the RNA-binding motif that promotes non-specific interactions with the tRNAs, it can also interact with the 5'-end of the enzyme's mRNA | Saccharomyces cerevisiae |
Synonyms | Comment | Organism |
---|---|---|
Aspartic acid translase | - |
Saccharomyces cerevisiae |
Aspartyl ribonucleate synthetase | - |
Saccharomyces cerevisiae |
aspartyl ribonuleic synthetase | - |
Saccharomyces cerevisiae |
Aspartyl-transfer ribonucleic acid synthetase | - |
Saccharomyces cerevisiae |
Aspartyl-transfer RNA synthetase | - |
Saccharomyces cerevisiae |
Aspartyl-tRNA synthetase | - |
Saccharomyces cerevisiae |
AspRS | - |
Saccharomyces cerevisiae |