Crystallization (Comment) | Organism |
---|---|
purified enzyme complexed with tRNAAsp from Thermus thermophilus or Escherichia coli, potential intermediate of the recognition process, protein solution: 15 mg/ml of enzyme, 7.35 mg/ml of tRNA, plus equal volume of reservoir solution: 10 mM MgCl2, 50 mM HEPES, pH 7.5, 0.7 M sodium citrate, 17°C, 2 weeks, X-ray diffraction structure determination at 3.5 A resolution, structure analysis | Thermus thermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | very low kinetic effiency at 17°C | Thermus thermophilus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-aspartate + tRNAAsp | Thermus thermophilus | - |
AMP + diphosphate + L-aspartyl-tRNAAsp | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus thermophilus | - |
prokaryotic dimeric class IIb aspartyl-tRNA synthetase | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + L-aspartate + tRNAAsp = AMP + diphosphate + L-aspartyl-tRNAAsp | pathway for tRNA binding and recognition is proposed | Thermus thermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-aspartate + tRNAAsp | - |
Thermus thermophilus | AMP + diphosphate + L-aspartyl-tRNAAsp | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Aspartic acid translase | - |
Thermus thermophilus |
Aspartyl ribonucleate synthetase | - |
Thermus thermophilus |
aspartyl ribonuleic synthetase | - |
Thermus thermophilus |
Aspartyl-transfer ribonucleic acid synthetase | - |
Thermus thermophilus |
Aspartyl-transfer RNA synthetase | - |
Thermus thermophilus |
Aspartyl-tRNA synthetase | - |
Thermus thermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
very low kinetic effiency at 17°C | Thermus thermophilus |