Cloned (Comment) | Organism |
---|---|
expression as His-tagged protein in Escherichia coli | Methanothermobacter thermautotrophicus |
expression as His-tagged protein in Escherichia coli | Thermococcus kodakarensis |
expression as His-tagged protein in Escherichia coli | Ferroplasma acidarmanus |
expression in Escherichia coli | Thermococcus kodakarensis |
expression in Escherichia coli | Ferroplasma acidarmanus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-aspartate + tRNAAsn | Methanothermobacter thermautotrophicus | the archaeal AspRS enzyme is nondiscriminating, which means that it forms Asp-tRNAAsp and Asp-tRNAAsn, which is the intermediate in AsntRNAAsn generation by ASp-tRNAAsn amidotransferase, in contrary bacterial enzymes are discriminating ones | AMP + diphosphate + L-aspartyl-tRNAAsn | - |
? | |
ATP + L-aspartate + tRNAAsp | Methanothermobacter thermautotrophicus | - |
AMP + diphosphate + L-aspartyl-tRNAAsp | - |
? | |
ATP + L-aspartate + tRNAAsp | Thermococcus kodakarensis | - |
AMP + diphosphate + L-aspartyl-tRNAAsp | - |
? | |
ATP + L-aspartate + tRNAAsp | Ferroplasma acidarmanus | - |
AMP + diphosphate + L-aspartyl-tRNAAsp | - |
? | |
additional information | Thermococcus kodakarensis | no acylation with L-aspartate of tRNAAsn, enzyme is discriminating, because it forms Asn-tRNAAsn by direct aminoacylation, not via the intermediate of Asp-tRNAAsn, and it contains no Asp-tRNAAsn amidotransferase | ? | - |
? | |
additional information | Ferroplasma acidarmanus | no acylation with L-aspartate of tRNAAsn, enzyme is discriminating, because it forms Asn-tRNAAsn by direct aminoacylation, not via the intermediate of Asp-tRNAAsn, and it contains no Asp-tRNAAsn amidotransferase | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ferroplasma acidarmanus | - |
- |
- |
Methanothermobacter thermautotrophicus | - |
- |
- |
Thermococcus kodakarensis | Q52428 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Thermococcus kodakarensis |
- |
Ferroplasma acidarmanus |
recombinant His-tagged enzyme from Escherichia coli | Methanothermobacter thermautotrophicus |
recombinant His-tagged enzyme from Escherichia coli | Thermococcus kodakarensis |
recombinant His-tagged enzyme from Escherichia coli | Ferroplasma acidarmanus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-aspartate + tRNAAsn | - |
Methanothermobacter thermautotrophicus | AMP + diphosphate + L-aspartyl-tRNAAsn | - |
? | |
ATP + L-aspartate + tRNAAsn | the archaeal AspRS enzyme is nondiscriminating, which means that it forms Asp-tRNAAsp and Asp-tRNAAsn, which is the intermediate in AsntRNAAsn generation by ASp-tRNAAsn amidotransferase, in contrary bacterial enzymes are discriminating ones | Methanothermobacter thermautotrophicus | AMP + diphosphate + L-aspartyl-tRNAAsn | - |
? | |
ATP + L-aspartate + tRNAAsp | - |
Methanothermobacter thermautotrophicus | AMP + diphosphate + L-aspartyl-tRNAAsp | - |
? | |
ATP + L-aspartate + tRNAAsp | - |
Thermococcus kodakarensis | AMP + diphosphate + L-aspartyl-tRNAAsp | - |
? | |
ATP + L-aspartate + tRNAAsp | - |
Ferroplasma acidarmanus | AMP + diphosphate + L-aspartyl-tRNAAsp | - |
? | |
ATP + L-aspartate + tRNAAsp | no activity with tRNAAsn | Thermococcus kodakarensis | AMP + diphosphate + L-aspartyl-tRNAAsp | - |
? | |
ATP + L-aspartate + tRNAAsp | no activity with tRNAAsn | Ferroplasma acidarmanus | AMP + diphosphate + L-aspartyl-tRNAAsp | - |
? | |
additional information | no acylation with L-aspartate of tRNAAsn | Thermococcus kodakarensis | ? | - |
? | |
additional information | no acylation with L-aspartate of tRNAAsn | Ferroplasma acidarmanus | ? | - |
? | |
additional information | no acylation with L-aspartate of tRNAAsn, enzyme is discriminating, because it forms Asn-tRNAAsn by direct aminoacylation, not via the intermediate of Asp-tRNAAsn, and it contains no Asp-tRNAAsn amidotransferase | Thermococcus kodakarensis | ? | - |
? | |
additional information | no acylation with L-aspartate of tRNAAsn, enzyme is discriminating, because it forms Asn-tRNAAsn by direct aminoacylation, not via the intermediate of Asp-tRNAAsn, and it contains no Asp-tRNAAsn amidotransferase | Ferroplasma acidarmanus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Aspartic acid translase | - |
Methanothermobacter thermautotrophicus |
Aspartic acid translase | - |
Thermococcus kodakarensis |
Aspartic acid translase | - |
Ferroplasma acidarmanus |
Aspartyl ribonucleate synthetase | - |
Methanothermobacter thermautotrophicus |
Aspartyl ribonucleate synthetase | - |
Thermococcus kodakarensis |
Aspartyl ribonucleate synthetase | - |
Ferroplasma acidarmanus |
aspartyl ribonuleic synthetase | - |
Methanothermobacter thermautotrophicus |
aspartyl ribonuleic synthetase | - |
Thermococcus kodakarensis |
aspartyl ribonuleic synthetase | - |
Ferroplasma acidarmanus |
Aspartyl-transfer ribonucleic acid synthetase | - |
Methanothermobacter thermautotrophicus |
Aspartyl-transfer ribonucleic acid synthetase | - |
Thermococcus kodakarensis |
Aspartyl-transfer ribonucleic acid synthetase | - |
Ferroplasma acidarmanus |
Aspartyl-transfer RNA synthetase | - |
Methanothermobacter thermautotrophicus |
Aspartyl-transfer RNA synthetase | - |
Thermococcus kodakarensis |
Aspartyl-transfer RNA synthetase | - |
Ferroplasma acidarmanus |
Aspartyl-tRNA synthetase | - |
Methanothermobacter thermautotrophicus |
Aspartyl-tRNA synthetase | - |
Thermococcus kodakarensis |
Aspartyl-tRNA synthetase | - |
Ferroplasma acidarmanus |
AspRS | - |
Methanothermobacter thermautotrophicus |
discriminating aspartyl-tRNA synthetase | - |
Thermococcus kodakarensis |
discriminating aspartyl-tRNA synthetase | - |
Ferroplasma acidarmanus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Ferroplasma acidarmanus |
60 | - |
- |
Thermococcus kodakarensis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | 60 | - |
Methanothermobacter thermautotrophicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Thermococcus kodakarensis |
6 | - |
- |
Ferroplasma acidarmanus |
7 | - |
assay at | Methanothermobacter thermautotrophicus |