Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 6.1.1.11 extracted from

  • Viswanathan, S.; Dignam, J.D.
    Seryl-tRNA synthetase from Bombyx mori. Purification and properties (1988), J. Biol. Chem., 263, 535-541.
    View publication on PubMed
Search for more literature for 6.1.1.11

General Stability

General Stability Organism
glycerol and DTT enhance stability of the enzyme Bombyx mori
not particularly susceptible to inactivation from by freezing and thawing Bombyx mori

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00025
-
 tRNASer substrate from Bombyx mori Bombyx mori
0.00026
-
 tRNASer substrate from rabbit liver Bombyx mori
0.11
-
 ATP
-
 Bombyx mori

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Bombyx mori
Mg2+ optimum concentration: 6 mM Bombyx mori

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
 2 * 60000, SDS-PAGE Bombyx mori
124000
-
 gel filtration, sedimentation velocity Bombyx mori

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Bombyx mori high abundance in middle silk gland may result from an adaption of this organ for the production of the serine-rich protein, sericin ?
-
 ?

Organism

Organism UniProt Comment Textmining
Bombyx mori
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bombyx mori

Source Tissue

Source Tissue Comment Organism Textmining
silk gland middle, high abundance in this organ may result from an adaption of this organ to the production of the serine-rich protein, sericin Bombyx mori
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
10.87
-
-
 Bombyx mori

Storage Stability

Storage Stability Organism
4°C, 50 mM potassium phosphate buffer, pH 7.5, 20% glycerol, 5 mM magnesium acetate, 0.2 mM EDTA, 0.5 mM DTT, 0.5 mM PMSF, 1 week, less than 20% loss of activity Bombyx mori

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-serine + tRNASer
-
 Bombyx mori AMP + diphosphate + L-seryl-tRNASer
-
 ?
additional information high abundance in middle silk gland may result from an adaption of this organ for the production of the serine-rich protein, sericin Bombyx mori ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 60000, SDS-PAGE Bombyx mori

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
 Hepes or Tris-HCl buffer Bombyx mori