Protein Variants | Comment | Organism |
---|---|---|
additional information | overexpressing, mutant lacking those 60 base pairs that encode the C-terminal peptide. The truncation mutant is less stable to heat inactivation at 42°C and binds tRNA with 3.6fold higher affinity, while the Km for Ser is 4fold increased relatively to the wild-type | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00005 | - |
tRNASer | aminoacylation, truncated enzyme | Saccharomyces cerevisiae | |
0.00019 | - |
tRNASer | aminoacylation, wild-type enzyme | Saccharomyces cerevisiae | |
0.0143 | - |
ATP | aminoacylation, truncated enzyme | Saccharomyces cerevisiae | |
0.021 | - |
ATP | aminoacylation, wild-type enzyme | Saccharomyces cerevisiae | |
0.0625 | - |
Ser | aminoacylation, wild-type enzyme | Saccharomyces cerevisiae | |
0.252 | - |
Ser | aminoacylation, truncated enzyme | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
overexpression, wild-type and mutant lacking the 60 base pairs encoding the C-terminal peptide | - |
Purification (Comment) | Organism |
---|---|
- |
Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-serine + tRNASer | - |
Saccharomyces cerevisiae | AMP + diphosphate + L-seryl-tRNASer | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
42 | - |
30 min, about 80% loss of activity of full-length enzyme, about 5% loss of activity of truncated enzyme | Saccharomyces cerevisiae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.41 | - |
tRNASer | Ser, , aminoacylation, truncated enzyme | Saccharomyces cerevisiae | |
0.5 | - |
Ser | ATP, , aminoacylation, wild-type enzyme | Saccharomyces cerevisiae | |
0.52 | - |
ATP | aminoacylation, truncated enzyme | Saccharomyces cerevisiae | |
0.55 | - |
tRNASer | aminoacylation, wild-type enzyme | Saccharomyces cerevisiae |