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Literature summary for 6.1.1.11 extracted from
- Identification of amino acids in the N-terminal domain of atypical methanogenic-type Seryl-tRNA synthetase critical for tRNA recognition (2009), J. Biol. Chem., 284, 30643-30651.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| the seryl-tRNA synthetase expression vector pET15bmMbSerRs is utilized, SerRS proteins are expressed in Escherichia coli cells | Methanosarcina barkeri |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| K141A | mutated enzyme, serylation rate lowered | Methanosarcina barkeri |
| K79A | mutated enzyme, significant drop in the serylation rate | Methanosarcina barkeri |
| K87A | mutated enzyme, retains the serylation ability comparable with the wild-type enzyme | Methanosarcina barkeri |
| K88A | mutated enzyme, retains the serylation ability comparable with the wild-type enzyme | Methanosarcina barkeri |
| K90A | mutated enzyme, retains the serylation ability comparable with the wild-type enzyme | Methanosarcina barkeri |
| N142A | mutated enzyme, serylation rate lowered | Methanosarcina barkeri |
| R143A | mutated enzyme, serylation rate lowered | Methanosarcina barkeri |
| R38A | mutated enzyme, serylation rate lowered | Methanosarcina barkeri |
| R76A | mutated enzyme, serylation ability completely lost | Methanosarcina barkeri |
| R78A | mutated enzyme, retains the serylation ability comparable with the wild-type enzyme | Methanosarcina barkeri |
| R94A | mutated enzyme, significant drop in the serylation rate | Methanosarcina barkeri |
| Y89A | mutated enzyme, retains the serylation ability comparable with the wild-type enzyme | Methanosarcina barkeri |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-serine + tRNASer | Methanosarcina barkeri | - |
AMP + diphosphate + L-seryl-tRNASer | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Methanosarcina barkeri | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| by affinity chromatography on nickel-nitrilotriacetc acid-agarose and cation exchange chromatography on a Resource S column | Methanosarcina barkeri |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-serine + tRNASer | - |
Methanosarcina barkeri | AMP + diphosphate + L-seryl-tRNASer | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| methanogenic-type seryl-tRNA synthetase | - |
Methanosarcina barkeri |
| mMbSerRS | - |
Methanosarcina barkeri |
| SerRS | - |
Methanosarcina barkeri |
| Seryl-tRNA synthetase | - |
Methanosarcina barkeri |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
aminoacylation assay | Methanosarcina barkeri |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
aminoacylation assay | Methanosarcina barkeri |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Methanosarcina barkeri | |
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Release 2023.1 (January 2023)
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