Cloned (Comment) | Organism |
---|---|
into the vectors pET30a+ and pUC19 | Streptomyces sp. |
into the vectors pET30a+, pSUP and pUC19, the H270G PCR product is cloned into the vector pET15b | Streptomyces sp. |
Protein Variants | Comment | Organism |
---|---|---|
H270G | SerRS2 mutant | Streptomyces sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
SB-217452 | - |
Streptomyces sp. | |
serine hydroxamate | - |
Streptomyces sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0086 | - |
tRNASer | 37°C, aminoacylation | Streptomyces sp. | |
0.012 | - |
tRNASer | 30°C, aminoacylation | Streptomyces sp. | |
0.24 | - |
tRNASer | aminoacylation | Streptomyces sp. | |
0.26 | - |
L-serine | diphosphate exchange | Streptomyces sp. | |
0.4 | - |
L-serine | diphosphate exchange | Streptomyces sp. | |
0.41 | - |
tRNASer | H270G mutant, aminoacylation | Streptomyces sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-serine + tRNASer | Streptomyces sp. | - |
AMP + diphosphate + L-seryl-tRNASer | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces sp. | D0UD38 | strain ATCC 700974 | - |
Streptomyces sp. | D0UD39 | strain ATCC 700974 | - |
Purification (Comment) | Organism |
---|---|
carried out with Ni-nitriloacetic acid spin columns | Streptomyces sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-serine + tRNASer | - |
Streptomyces sp. | AMP + diphosphate + L-seryl-tRNASer | - |
? |
Synonyms | Comment | Organism |
---|---|---|
SerRS1 | - |
Streptomyces sp. |
SerRS2 | - |
Streptomyces sp. |
seryl-tRNA synthetase 1 | - |
Streptomyces sp. |
seryl-tRNA synthetase 2 | - |
Streptomyces sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
aminoacylation assay | Streptomyces sp. |
37 | - |
aminoacylation assay | Streptomyces sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.057 | - |
tRNASer | 30°C, aminoacylation | Streptomyces sp. | |
0.125 | - |
tRNASer | 37°C, aminoacylation | Streptomyces sp. | |
0.18 | - |
tRNASer | H270G mutant, aminoacylation | Streptomyces sp. | |
1.37 | - |
tRNASer | aminoacylation | Streptomyces sp. | |
1.8 | - |
L-serine | diphosphate exchange | Streptomyces sp. | |
7 | - |
L-serine | diphosphate exchange | Streptomyces sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
aminoacylation assay | Streptomyces sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Streptomyces sp. |