Activating Compound | Comment | Organism | Structure |
---|---|---|---|
tRNASer | binding to the enzyme enhances the discrimination of the amino acid substrate and the activity, and diminishes the rate of misactivation of threonine, all due to a conformational chage in the enzymes active site | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
E281D/G291A | reduced activity compared to the wild-type enzyme | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetic changes in presence of several tRNAs, nonchargeable and heterologous ones, in the ATP-diphosphate exchange assay | Saccharomyces cerevisiae | |
0.0013 | - |
ATP | ATP-diphosphate exchange assay, wild-type enzyme, pH 7.2, 30°C, in presence of tRNASer | Saccharomyces cerevisiae | |
0.5 | - |
L-serine | ATP-diphosphate exchange assay, wild-type enzyme, pH 7.2, 30°C, in absence of tRNASer | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-serine + tRNASer | Saccharomyces cerevisiae | - |
AMP + diphosphate + L-seryl-tRNASer | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
purified recombinant wild-type and mutant enzyme | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + L-serine + tRNASer = AMP + diphosphate + L-seryl-tRNASer | tRNA substrate discrimination mechanism | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-serine + tRNASer | - |
Saccharomyces cerevisiae | AMP + diphosphate + L-seryl-tRNASer | - |
? | |
ATP + L-serine + tRNASer | wild-type and mutant tRNASer substrates, the enzyme complexed with 1 molecule of tRNASer is more specific and more efficient in catalyzing seryl-adenylate formation than the apoenzyme alone | Saccharomyces cerevisiae | AMP + diphosphate + L-seryl-tRNASer | - |
? | |
additional information | about 0.4% misactivation of L-threonine by the enzyme compared to cognate L-serine, rate is decreased by binding of the tRNASer, the enzyme also performs the ATP-diphosphate exchange reaction | Saccharomyces cerevisiae | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
62 kDa RNA-binding protein | - |
Saccharomyces cerevisiae |
Serine transfer RNA synthetase | - |
Saccharomyces cerevisiae |
Serine translase | - |
Saccharomyces cerevisiae |
Serine--tRNA ligase | - |
Saccharomyces cerevisiae |
SerRS | - |
Saccharomyces cerevisiae |
SerRSmt | - |
Saccharomyces cerevisiae |
SERSEC | - |
Saccharomyces cerevisiae |
Seryl-transfer ribonucleate synthetase | - |
Saccharomyces cerevisiae |
Seryl-transfer ribonucleic acid synthetase | - |
Saccharomyces cerevisiae |
Seryl-transfer RNA synthetase | - |
Saccharomyces cerevisiae |
Seryl-tRNA synthetase | - |
Saccharomyces cerevisiae |
Synthetase, seryl-transfer ribonucleate | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Saccharomyces cerevisiae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | changes in kcat in presence of several tRNAs, nonchargeable and heterologous ones, in the ATP-diphosphate exchange assay | Saccharomyces cerevisiae | |
3.9 | - |
ATP | ATP-diphosphate exchange assay, wild-type enzyme, pH 7.2, 30°C, in absence of tRNASer | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
assay at | Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | binding to the enzyme is not mediated by tRNA | Saccharomyces cerevisiae |