Crystallization (Comment) | Organism |
---|---|
monomeric MetRS in complex with the ligands methionine and adenosine or with methionine, X-ray diffraction structure determination and analysis at 2.3 A and 2.8 A resolution, respectively | Mycolicibacterium smegmatis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Mycolicibacterium smegmatis | |
Zn2+ | binding domain and binding structure, overview | Mycolicibacterium smegmatis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-methionine + tRNAMet | Mycolicibacterium smegmatis | - |
AMP + diphosphate + L-methionyl-tRNAMet | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycolicibacterium smegmatis | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-methionine + tRNAMet | - |
Mycolicibacterium smegmatis | AMP + diphosphate + L-methionyl-tRNAMet | - |
? | |
ATP + L-methionine + tRNAMet | analysis of methionine and adenosine binding to MetRS, catalytic domain structure, overview. The KMSKS domain, residues 293-350, or stem contact fold domain, with a beta-alpha-alpha-beta-alpha topology domain, connects the catalytic domain with the anticodon domain, KMSKS domain conformation and structure, modelling, overview | Mycolicibacterium smegmatis | AMP + diphosphate + L-methionyl-tRNAMet | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | MetRs is a monomeric multidomain enzyme, comparisons with other MetRS structures. The MetRS core unit consists of four domains: the catalytic domain, the CP domain, the KMSKS domain, and an anticodon-binding domain.the enzyme crystal structure contains a KMSKS(301-305) loop in proximity to the catalytic site in an open and inactive conformation, overview | Mycolicibacterium smegmatis |
Synonyms | Comment | Organism |
---|---|---|
methionyl-tRNA synthetase | - |
Mycolicibacterium smegmatis |
MetRS | - |
Mycolicibacterium smegmatis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | binding structure, overview | Mycolicibacterium smegmatis |