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Literature summary for 6.1.1.10 extracted from

  • Crepin, T.; Schmitt, E.; Mechulam, Y.; Sampson, P.B.; Vaughan, M.D.; Honek, J.F.; Blanquet, S.
    Use of analogues of methionine and methionyl adenylate to sample conformational changes during catalysis in Escherichia coli methionyl-tRNA synthetase (2003), J. Mol. Biol., 332, 59-72.
    View publication on PubMed
Search for more literature for 6.1.1.10

Crystallization (Commentary)

Crystallization (Comment) Organism
crystals of purified free enzyme and enzyme complexed with L-methionine are diffused by several methionine analogues, i.e. L-difluoromethionine, L-trifluoromethionine, D,L-phosphomethionine and D,L-iodo-methionine, X-ray diffraction structure determination at 1.9 A resolution and analysis Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-methionine + tRNAMet Escherichia coli
-
 AMP + diphosphate + L-methionyl-tRNAMet
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli P00959
-
-

Reaction

Reaction Comment Organism Reaction ID
ATP + L-methionine + tRNAMet = AMP + diphosphate + L-methionyl-tRNAMet substrate binding and mechanism Escherichia coli
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-methionine + tRNAMet
-
 Escherichia coli AMP + diphosphate + L-methionyl-tRNAMet
-
 ?
ATP + L-methionine + tRNAMet binding of L-methionine induces conformational changes of the active site of the enzyme, amino acid residues Y15 and W253 are important for the strength of binding, H301 is responsible for the specific recognition of the sulfur atom of methionine Escherichia coli AMP + diphosphate + L-methionyl-tRNAMet
-
 ?

Synonyms

Synonyms Comment Organism
Methionine translase
-
 Escherichia coli
Methionine--tRNA ligase
-
 Escherichia coli
Methionyl tRNA synthetase
-
 Escherichia coli
Methionyl-transfer ribonucleate synthetase
-
 Escherichia coli
Methionyl-transfer ribonucleic acid synthetase
-
 Escherichia coli
Methionyl-transfer RNA synthetase
-
 Escherichia coli
MetRS
-
 Escherichia coli
Synthetase, methionyl-transfer ribonucleate
-
 Escherichia coli

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Escherichia coli