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Literature summary for 6.1.1.10 extracted from
- Methionine-tRNA-ligase from wheat germ. Purification and properties (1975), FEBS Lett., 56, 268-272.
General Stability
| General Stability | Organism |
|---|---|
| Mg2+, required for stability, enzyme loses 80% of its activity when stored at -20°C for 3 days in buffer lacking Mg2+ | Triticum aestivum |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| K+ | in presence of 15 mM Mg2+, K+ stimulates up to concentration of about 50 mM, inhibits at higher concentrations. Unable to stimulate aminoacylation in absence of Mg2+ | Triticum aestivum |  |
| Mg2+ | 2 (ATP-diphosphate exchange), 1.5 (aminoacylation) | Triticum aestivum | |
| Mn2+ | optimal Mn2+/ATP ratio: 1 (ATP-diphosphate exchange), 1.5 (aminoacylation) | Triticum aestivum | |
| NH4+ | in presence of 15 mM Mg2+, NH4+ stimulates up to a concentration of about 50 mM, inhibits at higher concentrations. In presence of 0.5 mM Mg2+, NH4+ alone stimulates aminoacylation | Triticum aestivum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 74000 | - |
2 * 74000, SDS-PAGE | Triticum aestivum |
| 165000 | - |
gel filtration | Triticum aestivum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Triticum aestivum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Triticum aestivum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| germ | - |
Triticum aestivum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.087 | - |
- |
Triticum aestivum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-methionine + tRNAMet | - |
Triticum aestivum | AMP + diphosphate + L-methionyl-tRNAMet | - |
? | |
| additional information | methionine-dependent ATP-diphosphate exchange | Triticum aestivum | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 74000, SDS-PAGE | Triticum aestivum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | 7.9 | ATP-diphosphate exchange | Triticum aestivum |
| 8.1 | 8.4 | aminoacylation | Triticum aestivum |
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