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Literature summary for 6.1.1.1 extracted from

  • Zhang, D.; Vaidehi, N.; Goddard, W.A.3rd.; Danzer, J.F.; Debe, D.
    Structure-based design of mutant Methanococcus jannaschii tyrosyl-tRNA synthetase for incorporation of O-methyl-L-tyrosine (2002), Proc. Natl. Acad. Sci. USA, 99, 6579-6584.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.1.1.1

Application

Application Comment Organism
biotechnology incorporation of unusual specific amino acids into proteins in in vitro translation systems by mutant enzyme with altered substrate specificity. e.g. mutant Y32Q/D158A Methanocaldococcus jannaschii

Protein Variants

Protein Variants Comment Organism
additional information computational design of possible mutants with altered substrate specificity by prediction of the binding structure and calculation of binding energies of mutants to amino acids, overview Methanocaldococcus jannaschii
Y32Q/D158A site-directed mutagenesis, enzyme mutant discriminates between L-tyrosine and O-methyl-L-tyrosine, with a high activity only with the latter Methanocaldococcus jannaschii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-tyrosine + tRNATyr Methanocaldococcus jannaschii
-
 AMP + L-Tyr-tRNATyr + diphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Methanocaldococcus jannaschii Q57834
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-tyrosine + tRNATyr
-
 Methanocaldococcus jannaschii AMP + L-Tyr-tRNATyr + diphosphate
-
 ?
ATP + O-methyl-L-tyrosine + tRNATyr
-
 Methanocaldococcus jannaschii AMP + O-methyl-L-Tyr-tRNATyr + diphosphate
-
 ?

Synonyms

Synonyms Comment Organism
Tyrosyl-tRNA synthetase
-
 Methanocaldococcus jannaschii
TyrRS
-
 Methanocaldococcus jannaschii