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Literature summary for 6.1.1.1 extracted from

  • Fechter, P.; Rudinger-Thirion, J.; Theobald-Dietrich, A.; Giege, R.
    Identity of tRNA for yeast tyrosyl-tRNA synthetase: tyrosylation is more sensitive to identity nucleotides than to structural features (2000), Biochemistry, 39, 1725-1733.
    View publication on PubMed
Search for more literature for 6.1.1.1

Protein Variants

Protein Variants Comment Organism
additional information construction of diverse tRNA variants of the native tRNATyr from yeast by heterologous in vitro translation, transplantation and point mutations Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetics, influence of assay conditions, Km for diverse tRNA variants, overview Saccharomyces cerevisiae
0.002
-
 native yeast tRNATyr pH 7.5, 30°C Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + tyrosine + tRNATyr Saccharomyces cerevisiae
-
 AMP + Tyr-tRNATyr + diphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-tyrosine + native yeast tRNATyr
-
 Saccharomyces cerevisiae AMP + diphosphate + L-tyrosyl-tRNATyr
-
 ?
ATP + tyrosine + tRNATyr
-
 Saccharomyces cerevisiae AMP + Tyr-tRNATyr + diphosphate
-
 ?
ATP + tyrosine + tRNATyr heterologous tRNATyr from Escherichia coli and Methanococcus jannashii, tyrosylation efficiency of tRNA variants: determinants are base pair C1-G72, discriminator residue A73, and the 3 anticodon bases G34, U35, and A36 Saccharomyces cerevisiae AMP + Tyr-tRNATyr + diphosphate
-
 ?
additional information no activity with tRNAAsp and tRNAPhe Saccharomyces cerevisiae ?
-
 ?

Synonyms

Synonyms Comment Organism
Tyrosyl-tRNA synthetase
-
 Saccharomyces cerevisiae
TyrRS
-
 Saccharomyces cerevisiae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
 additional information influence of assay conditions, kcat for diverse tRNA variants, overview Saccharomyces cerevisiae
1.5
-
 native yeast tRNATyr pH 7.5, 30°C Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Saccharomyces cerevisiae