Activating Compound | Comment | Organism | Structure |
---|---|---|---|
glutathione | activates above 0.15 mM | Pseudomonas sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | - |
Pseudomonas sp. | |
Hg2+ | - |
Pseudomonas sp. | |
iodoacetic acid | - |
Pseudomonas sp. | |
monoiodoacetate | - |
Pseudomonas sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.053 | - |
2-hydroxy-2H-chromene-2-carboxylate | pH 7.4, 30°C | Pseudomonas sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25000 | - |
1 * 25000, SDS-PAGE | Pseudomonas sp. |
27000 | - |
gel filtration | Pseudomonas sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | - |
- |
- |
Pseudomonas sp. TA-2 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
62 | - |
- |
Pseudomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-hydroxy-2H-chromene-2-carboxylate | the product trans-o-hydroxybenzylidenepyruvate is analyzed by 1H NMR spectrum | Pseudomonas sp. | (3E)-4-(2-hydroxyphenyl)-2-oxobut-3-enoate | i.e. (E)-2'-hydroxybenzylidenepyruvate, i.e. trans-o-hydroxybenzylidenepyruvate | ? | |
2-hydroxy-2H-chromene-2-carboxylate | the product trans-o-hydroxybenzylidenepyruvate is analyzed by 1H NMR spectrum | Pseudomonas sp. TA-2 | (3E)-4-(2-hydroxyphenyl)-2-oxobut-3-enoate | i.e. (E)-2'-hydroxybenzylidenepyruvate, i.e. trans-o-hydroxybenzylidenepyruvate | ? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 25000, SDS-PAGE | Pseudomonas sp. |
Synonyms | Comment | Organism |
---|---|---|
2HC2CA isomerase | - |
Pseudomonas sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
8 | - |
- |
Pseudomonas sp. |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
6.8 | 9.5 | pH 6.8: about 35% of maximal activity, pH 9.5: about 40% of maximal activity | Pseudomonas sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
10 min, stable at temperature up to | Pseudomonas sp. |
50 | - |
10 min, in presence of glutathione at 2.5 mM, the enzyme is stable up to | Pseudomonas sp. |
55 | - |
10 min, 13% of the original activity remains. In presence of glutathione at 2.5 mM, 68% of the original activity remains after 10 min | Pseudomonas sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
1.5 | 10 | 4°C, 40 h, stable in the range of pH 1.5 to 10.0 | Pseudomonas sp. |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Pseudomonas sp. | isoelectric focusing | - |
5 |