Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pseudomonas putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | Q51948 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-hydroxy-2H-chromene-2-carboxylate | extracts of Escherichia coli JM109 carrying pRE718 catalyze the conversion of trans-o-hydroxybenzylidenepyruvate or 2-hydroxychromene-2-carboxylate to an equilibrium mixture that contained 55% 2-hydroxychromene-2-carboxylate and 45% trans-o-hydroxybenzylidenepyruvate at pH 7. At pH 10 the reaction occus entirely in on direction, the conversion of 2-hydroxychromene-2-carboxylate to trans-o-hydroxybenzylidenepyruvate. The product is identified by nuclear magnetic resonance spectroscopy. This isomerization occurs spontaneously, although at a slower rate than the enzyme-catalyzed reaction | Pseudomonas putida | (3E)-4-(2-hydroxyphenyl)-2-oxobut-3-enoate | i.e. (E)-2'-hydroxybenzylidenepyruvate, i.e. trans-o-hydroxybenzylidenepyruvate | r |
Synonyms | Comment | Organism |
---|---|---|
2-hydroxychromene-2-carboxylate isomerase | - |
Pseudomonas putida |
HCCA isomerase | - |
Pseudomonas putida |