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Literature summary for 5.6.1.3 extracted from

  • Umezu, N.; Umeki, N.; Mitsui, T.; Kondo, K.; Maruta, S.
    Characterization of a novel rice kinesin O12 with a calponin homology domain (2011), J. Biochem., 149, 91-101.
    View publication on PubMed
Search for more literature for 5.6.1.3

Cloned(Commentary)

Cloned (Comment) Organism
C-terminal motor domain (amino acid residues 372-717) expressed in Escherichia coli BL21(DE3) Oryza sativa Japonica Group

Inhibitors

Inhibitors Comment Organism Structure
actin inhibition in the presence of microtubles Oryza sativa Japonica Group

Organism

Organism UniProt Comment Textmining
Oryza sativa Japonica Group AK065586
-
-

Purification (Commentary)

Purification (Comment) Organism
immobilized metal ion affinity chromatography (Co2+) Oryza sativa Japonica Group

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 1.19 mol phosphate/kinesin mol/min in the absence of microtubles, 90.6 mol phosphate/kinesin mol/min in the presence of microtubles, Vmax/2 at 0.0037 mM microtubles, pH 7.5, 25°C Oryza sativa Japonica Group

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + a kinesin associated with a microtubule at position n
-
 Oryza sativa Japonica Group ADP + phosphate + a kinesin associated with a microtubule at position n+1 (toward the plus end)
-
 ?

Synonyms

Synonyms Comment Organism
kinesin O12 classified as kinesin-14 family member Oryza sativa Japonica Group

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
 in the presence of microtubles Oryza sativa Japonica Group

pH Range

pH Minimum pH Maximum Comment Organism
5.5 9 in the presence of microtubles Oryza sativa Japonica Group