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Literature summary for 5.5.1.26 extracted from

  • Sultana, A.; Kallio, P.; Jansson, A.; Wang, J.S.; Niemi, J.; Mäntsälä, P.; Schneider, G.
    Structure of the polyketide cyclase SnoaL reveals a novel mechanism for enzymatic aldol condensation (2004), EMBO J., 23, 1911-1921.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 5.5.1.26

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Streptomyces nogalater

Crystallization (Commentary)

Crystallization (Comment) Organism
crystal structure of the enzyme with a bound product, determined to 1.35 A resolution Streptomyces nogalater

Protein Variants

Protein Variants Comment Organism
D121A inactive mutant enzyme Streptomyces nogalater
D121N mutant enzyme forms insoluble inclusion bodies Streptomyces nogalater
F15Y mutant enzyme shows 5% of the activity compared to the wild-type enzyme Streptomyces nogalater
Q105A mutant enzyme shows 16% of the activity compared to the wild-type enzyme Streptomyces nogalater
Q105E mutant enzyme forms insoluble inclusion bodies Streptomyces nogalater

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
methyl nogalonate Streptomyces nogalater
-
 nogalaviketone
-
 ?

Organism

Organism UniProt Comment Textmining
Streptomyces nogalater Q9RN59
-
-

Reaction

Reaction Comment Organism Reaction ID
nogalaviketone = methyl nogalonate the mechanism of the intramolecular aldol condensation catalysed by the enzyme is different from that of the classical aldolases, which employ covalent Schiff base formation or a metal ion cofactor. The invariant residue Asp121 acts as an acid/base catalyst during the reaction. Stabilisation of the enol(ate) intermediate is mainly achieved by the delocalisation of the electron pair over the extended p system of the substrate Streptomyces nogalater
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
methyl nogalonate
-
 Streptomyces nogalater nogalaviketone
-
 ?
methyl nogalonate the mechanism of the intramolecular aldol condensation catalysed by the enzyme is different from that of the classical aldolases, which employ covalent Schiff base formation or a metal ion cofactor. The invariant residue Asp121 acts as an acid/base catalyst during the reaction. Stabilisation of the enol(ate) intermediate is mainly achieved by the delocalisation of the electron pair over the extended p system of the substrate Streptomyces nogalater nogalaviketone
-
 ?

Synonyms

Synonyms Comment Organism
methyl nogalonate cyclase
-
 Streptomyces nogalater
SnoaL
-
 Streptomyces nogalater

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Streptomyces nogalater